Phylogenetic positioning of Listeria ivanovii identified in aborted sheep in Kars Region (Turkey)
Yazarlar (11)
Doç. Dr. Doğan AKÇA
Kafkas Üniversitesi, Türkiye
Prof. Dr. Fatih BÜYÜK
Kafkas Üniversitesi, Türkiye
Dr. Öğr. Üyesi Elif ÇELİK
Kafkas Üniversitesi, Veteriner Fakültesi, Türkiye
Prof. Dr. Aliye GÜLMEZ SAĞLAM
Kafkas Üniversitesi, Veteriner Fakültesi, Türkiye
Prof. Dr. Salih OTLU
Kafkas Üniversitesi, Veteriner Fakültesi, Türkiye
Prof. Dr. Serpil DAĞ
Kafkas Üniversitesi, Veteriner Fakültesi, Türkiye
Prof. Dr. Özgür ÇELEBİ
Kafkas Üniversitesi, Veteriner Fakültesi, Türkiye
Dr. Öğr. Üyesi Mustafa Reha COŞKUN
Kafkas Üniversitesi, Veteriner Fakültesi, Türkiye
Arş. Gör. Eray BÜYÜK
Kafkas Üniversitesi, Veteriner Fakültesi, Türkiye
Doç. Dr. Emin KARAKURT
Kafkas Üniversitesi, Türkiye
Prof. Dr. Mitat ŞAHİN
Kafkas Üniversitesi, Veteriner Fakültesi, Türkiye
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale |
| Dergi Adı | Thai Journal of Veterinary Medicine |
| Dergi ISSN | 0125-6491 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2022 |
| Cilt No | 52 |
| Sayı | 1 |
| Sayfalar | 145 / 150 |
| DOI Numarası | 10.14456/tjvm.2022.14 |
| Makale Linki | https://he01.tci-thaijo.org/index.php/tjvm/article/view/255586/172620 |
| Özet |
| This study aimed to investigate Listeria species in various samples taken from aborted sheep in Kars province and to determine the position of emerged strains in the phylogenetic tree. Milk (n= 229) and vaginal swab (n= 263) samples from aborted sheep and abomasum contents (n= 46) of aborted sheep fetuses were investigated by the culture and Polymerase Chain Reaction (PCR) methods. For the phylogenetic analysis of isolates, the 16S rRNA gene region was sequenced. In the culture, the Listeria agent was not isolated from any of the vaginal swabs or milk samples. Among the 46 fetal abomasum contents, a Listeria suspicious colony was obtained from only one (2.17%) sample which belonged to an enterprise with a history of silage feeding. This isolate was identified as Listeria ivanovii by both conventional methods and genus and species-specific PCR. In addition, Listeria DNA was detected in a total of 8 (1.48%) samples (in 1 vaginal swab, 1 milk and 6 fetal abomasum samples) by direct genus-specific PCR. However, these samples could not be identified, except for one of the 6 abomasum content samples that was found as L. ivanovii. The L. ivanovii isolate was double-identified after the amplification of the 16S rRNA gene region followed by sequence analysis. Sequence analysis of the 16S rRNA gene region gave a phylogenetic position to the isolate, L. ivanovii, which had been scarcely identified from the abortive specimens. |
| Anahtar Kelimeler |
| 16S rRNA sequencing | culture | Listeria ivanovii | PCR | phylogeny |