| Yazarlar |
Prof. Dr. Özgür ÇELEBİ
Kafkas Üniversitesi, Türkiye |
Ümit Özdemir
Türkiye |
Prof. Dr. Fatih BÜYÜK
Kafkas Üniversitesi, Türkiye |
|
A Ünsal Baca
Türkiye |
|
Şh Erpek
Türkiye |
|
M Karahan
Türkiye |
Prof. Dr. Salih OTLU
Türkiye |
Prof. Dr. Mitat ŞAHİN
Türkiye |
Dr. Öğr. Üyesi Mustafa Reha COŞKUN
Türkiye |
Dr. Öğr. Üyesi Elif ÇELİK
Kafkas Üniversitesi, Türkiye |
Doç. Dr. Aliye GÜLMEZ SAĞLAM
Türkiye |
Arş. Gör. Eray BÜYÜK
Türkiye |
Doç. Dr. Doğan AKÇA
Türkiye |
| Özet |
| This study aimed to investigate Mycoplasma species in the lungs of 500 geese with pneumonia from the Kars region (Turkey) via cultural and molecular methods. The samples were cultured on Frey’s Broth and Agar media. To identify Mycoplasma species a Growth Inhibition Test was used. The identification was continued with species-specific PCR and sequence analysis which provide amplification of the genes dnaX, pcrA, rpoB, and the sequence of the 16S rRNA gene, respectively. In addition, Mycoplasma gallisepticum and Mycoplasma synoviae from pneumonic lung samples were directly analyzed via Multiplex Real-time PCR. As a result, 51 Mycoplasma strains were isolated and 32 were identified as Mycoplasma anatis, 9 as Mycoplasma anseris, 5 as Mycoplasma cloacale and 3 as Mycoplasma anserisalpingitis. Two Mycoplasma isolates that could not be identified were grouped in the same branch as a result of 16S RNA sequencing and their nearest neighbour was found to be Mycoplasma sp. 2045 (GenBankNo.MK615061.1). M. gallisepticum DNA was detected in 3 pneumonic lung samples and M. gallisepticum/M. synoviae DNAs were found simultaneously in 1 sample. While some Mycoplasma species identified in this study consolidated their place as pneumonic agents, some increased their potential to become a pneumonic agent when compared with cases caused by well-recognized Mycoplasma strains. Two isolates were identified as-Mycoplasma spp. as their 16S rRNA gene sequence identity levels scored below the threshold of 98.7% for species demarcation and still need to be defined whether they are possible representatives of a novel Mycoplasma species. |
| Anahtar Kelimeler |
| 16S rRNA sequencing | Culture | Goose | Mycoplasma spp | PCR |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | Braz. J. Poult. Sci. |
| Dergi ISSN | 1806-9061 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2022 |
| Cilt No | 24 |
| Sayı | 1 |
| Doi Numarası | 10.1590/1806-9061-2021-1522 |
| Makale Linki | https://www.scielo.br/j/rbca/a/PtsVSW8P7KPBGQg75qBvZWM/?format=pdf&lang=en |
| Atıf Sayıları |
