Molecular characterization of Bacillus anthracis isolates recovered from nomic and non-nomic hosts.
Yazarlar (15)
Doğan Akça Kafkas Üniversitesi, Türkiye
Prof. Dr. Fatih BÜYÜK Kafkas Üniversitesi, Türkiye
Emre Karakaya Erciyes Üniversitesi, Türkiye
Mustafa Reha Coşkun Kafkas Üniversitesi, Türkiye
Elif Çelik Kafkas Üniversitesi, Türkiye
Mitat Şahin Kafkas Üniversitesi, Türkiye
Özgür Çelebi Kafkas Üniversitesi, Türkiye
Salih Otlu Kafkas Üniversitesi, Türkiye
Aliye Gülmez Sağlam Kafkas Üniversitesi, Türkiye
Eray Büyük Kafkas Üniversitesi, Türkiye
Seda Durhan Kafkas Üniversitesi, Türkiye
Izzet Burçin Saticioğlu Erciyes Üniversitesi, Türkiye
Seçil Abay Erciyes Üniversitesi, Türkiye
Tuba Kayman University Of Health Sciences, Türkiye
Fuat Aydin Erciyes Üniversitesi, Türkiye
Makale Türü Açık Erişim Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı Turkish Journal of Veterinary and Animal Sciences
Dergi ISSN 1300-0128 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili Türkçe Basım Tarihi 01-2022
Cilt / Sayı / Sayfa 46 / 1 / 44–51 DOI 10.3906/vet-2106-111
Makale Linki https://journals.tubitak.gov.tr/cgi/viewcontent.cgi?article=4148&context=veterinary
Özet
Anthrax, which is primarily a disease of herbivores, is known to infect many animal species. In order to break the cycle of anthrax infection, it is important to identify the potential hosts or reservoirs of its causative agent. Therefore, this study aimed to characterize Bacillus anthracis isolates recovered from a variety of hosts and environments during anthrax sporadic in the Kars and Kayseri regions of Turkey. For this purpose, a total of 23 B. anthracis field isolates obtained from human, cattle, sheep, dog, horse, puma, soil, and fodder were used. In addition, the Sterne vaccine strain was included. All B. anthracis isolates were confirmed via molecular tests using plasmid-based PCR and 16S rRNA gene sequencing. All were found to be positive for protective antigen (PA) and capsule (Cap) genes. The presence of two specific 16S rRNA positions (1139 and 1148) with nucleotide mismatches was used to differentiate between B. anthracis species in the Bacillus cereus group, which has a relatively high (>99%) 16S rRNA sequence similarity. All of our isolates, including the Sterne vaccine strain, were classified in the same 16S rRNA type (Type 6) that has been reported previously as being the predominant type for B. anthracis isolates.
Anahtar Kelimeler
16S rRNA sequencing | Bacillus anthracis | Phylogeny | Plasmid-based PCR
BM Sürdürülebilir Kalkınma Amaçları
Atıf Sayıları
Web of Science 3
Scopus 3
Molecular characterization of Bacillus anthracis isolates recovered from nomic and non-nomic hosts.

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