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Diagnosis of pulmonary aspergillosis in geese by histopathological and microbiological methods   
Yazarlar
 Emin KARAKURT Emin KARAKURT
Türkiye
 Fatih BÜYÜK Fatih BÜYÜK
Kafkas Üniversitesi, Türkiye
 Serpil DAĞ Serpil DAĞ
Kafkas Üniversitesi, Türkiye
 Enver BEYTUT Enver BEYTUT
Kafkas Üniversitesi, Türkiye
 Özgür ÇELEBİ Özgür ÇELEBİ
Kafkas Üniversitesi, Türkiye
 Doğan AKÇA Doğan AKÇA
Kafkas Üniversitesi, Türkiye
Dr. Öğr. Üyesi Hilmi NUHOĞLU Dr. Öğr. Üyesi Hilmi NUHOĞLU
Kafkas Üniversitesi, Türkiye
Arş. Gör. Ayfer YILDIZ UYSAL Arş. Gör. Ayfer YILDIZ UYSAL
Kafkas Üniversitesi, Türkiye
Emre Kurtbaş
Türkiye
Özet
In this study, we aimed to determine the presence of pulmonary aspergillosis by histopathological and microbiological methods in geese that are economically grown in Kars region of Turkey. Totally 150 lung tissue samples of geese, an average age of 9 weeks, which died between 2013 and 2020 and were brought to our department were included in the study. Following the systemic necropsy paraffin blocks were prepared from the tissue samples and stained with Hematoxylin & Eosin (H&E) in order to detect histopathological changes. In order to reveal the presence of Aspergillus fungi, Periodic acid–Schiff (PAS) staining was applied to the sections as suggested by Facepath company. The microbiological examination of the tissue samples was carried out by the standard mycological culture technique on Sabouraud Dextrose Agar and by the phenotypical characterization of the emerged cultures. Various clinical symptoms such as difficulties in breathing, wheezing, weakness, anorexia, depression, diarrhea and nervous signs were recorded in geese. We observed large and small multifocal yellowish-white nodular structures in the lungs and air sacs macroscopically. In the histopathological examination of the lung tissues, we detected granulomatous structures with varying numbers and sizes. We diagnosed the Aspergillus agents in 20 (13.33%) of the tissue samples by detecting structures resembling typical tree branches in the middle of granulomatous structures with PAS staining. An identical positivity was obtained by the microbiological method and the emerged agent was solely identified as Aspergillus fumigatus with the growth pattern and macroscopic and microscopic morphological features. In conclusion, we found the presence of aspergillosis as 13.33% by histopathological and microbiological methods in geese which were brought to our department between 2013-2020. Based on these data, we concluded that aspergillosis is one of the most important infectious diseases among the goose deaths in Kars region of Turkey
Anahtar Kelimeler
Makale Türü Özgün Makale
Makale Alt Türü Ulusal alan endekslerinde (TR Dizin, ULAKBİM) yayımlanan tam makale
Dergi Adı Journal of Advances in VetBio Science and Techniques
Dergi ISSN 2548-1150
Dergi Tarandığı Indeksler TR DİZİN
Makale Dili İngilizce
Basım Tarihi 04-2021
Cilt No 6
Sayı 1
Sayfalar 15 / 21
Doi Numarası 10.31797/vetbio.853459
Makale Linki https://dergipark.org.tr/tr/download/article-file/1487345
BM Sürdürülebilir Kalkınma Amaçları
Atıf Sayıları
Diagnosis of pulmonary aspergillosis in geese by histopathological and microbiological methods

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