Protection of farm goats by combinations of recombinant peptides and formalin inactivated spores from a lethal Bacillus anthracis challenge under field conditions..

Koehler, Susanne M.; BÜYÜK, FATİH; Beyer, Wolfgang; Celebi, Ozgur; Demiraslan, Hayati; Doganay, Mehmet; Sahin, Mitat; Moehring, Jens; Ndumnego, Okechukwu C.; Otlu, Salih; Heerden, Henriette Van

doi:10.1186/s12917-017-1140-2

Protection of farm goats by combinations of recombinant peptides and formalin inactivated spores from a lethal Bacillus anthracis challenge under field conditions..

Yazarlar (11)

Susanne M. Koehler Universität Hohenheim, Almanya

Prof. Dr. Fatih BÜYÜK Kafkas Üniversitesi, Türkiye

Ozgur Celebi Kafkas Üniversitesi, Veteriner Fakültesi, Türkiye

Hayati Demiraslan Erciyes Üniversitesi, Türkiye

Mehmet Doganay Erciyes Üniversitesi, Türkiye

Mitat Sahin Kafkas Üniversitesi, Veteriner Fakültesi, Türkiye

Jens Moehring Universität Hohenheim, Almanya

Okechukwu C. Ndumnego University Of Pretoria, Güney Afrika Cumhuriyeti

Salih Otlu Kafkas Üniversitesi, Veteriner Fakültesi, Türkiye

Henriette Van Heerden University Of Pretoria, Güney Afrika Cumhuriyeti

Wolfgang Beyer Universität Hohenheim, Almanya

Makale Türü	Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı	BMC Veterinary Research
Dergi ISSN	1746-6148 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler	SCI-Expanded
Makale Dili	İngilizce	Basım Tarihi	01-2017
Cilt / Sayı / Sayfa	13 / 1 / –	DOI	10.1186/s12917-017-1140-2

Özet

Background: Bacillus (B.) anthracis, the causal agent of anthrax, is effectively controlled by the Sterne live spore vaccine (34F2) in animals. However, live spore vaccines are not suitable for simultaneous vaccination and antibiotic treatment of animals being at risk of infection in an outbreak situation. Non-living vaccines could close this gap. Results: In this study a combination of recombinant protective antigen and recombinant Bacillus collagen-like antigen (rBclA) with or without formalin inactivated spores (FIS), targeted at raising an immune response against both the toxins and the spore of B. anthracis, was tested for immunogenicity and protectiveness in goats. Two groups of goats received from local farmers of the Kars region of Turkey were immunized thrice in three weeks intervals and challenged together with non-vaccinated controls with virulent B. anthracis, four weeks after last immunization. In spite of low or none measurable toxin neutralizing antibodies and a surprisingly low immune response to the rBclA, 80% of the goats receiving the complete vaccine were protected against a lethal challenge. Moreover, the course of antibody responses indicates that a two-step vaccination schedule could be sufficient for protection. Conclusion: The combination of recombinant protein antigens and FIS induces a protective immune response in goats. The non-living nature of this vaccine would allow for a concomitant antibiotic treatment and vaccination procedure. Further studies should clarify how this vaccine candidate performs in a post infection scenario controlled by antibiotics.

Anahtar Kelimeler

Animal vaccine | Anthrax | Bacillus anthracis | Vaccination

BM Sürdürülebilir Kalkınma Amaçları

Atıf Sayıları
WoS	4
SCOPUS	6

Protection of farm goats by combinations of recombinant peptides and formalin inactivated spores from a lethal Bacillus anthracis challenge under field conditions..

Dergi Adı	BMC Veterinary Research
Yayıncı	BioMed Central Ltd
Açık Erişim	Evet
E-ISSN	1746-6148
CiteScore	4,3
SJR	0,723
SNIP	1,053

Protection of farm goats by combinations of recombinant peptides and formalin inactivated spores from a lethal Bacillus anthracis challenge under field conditions..

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