| Yazarlar |
Callum Cooper
Türkiye |
Prof. Dr. Fatih BÜYÜK
Kafkas Üniversitesi, Türkiye |
|
Bettına Schelkle
Türkiye |
Doç. Dr. Aliye GÜLMEZ SAĞLAM
Kafkas Üniversitesi, Türkiye |
Dr. Öğr. Üyesi Elif ÇELİK
Kafkas Üniversitesi, Türkiye |
Prof. Dr. Özgür ÇELEBİ
Kafkas Üniversitesi, Türkiye |
Prof. Dr. Mitat ŞAHİN
Kafkas Üniversitesi, Türkiye |
|
Tom Hawkyard
Türkiye |
|
Les Baıllıe
Türkiye |
| Özet |
| The Bacillus anthracis virulence plasmid pXO2, which encodes for a polypeptide capsule, can be lost during long term laboratory storage. To determine if pXO2 is lost in nature we screened B. anthracis isolates obtained from B. anthracis spores from contaminated animal burial sites in Turkey for their ability to express a capsule upon primary culture. A total of 672 B. anthracis colonies were examined of which ten produced a mixed mucoid (capsule +ve)/non-mucoid (capsule −ve) phenotype and a further one colony yielded non-mucoid colonies upon repeated culture. Screening by PCR using pXO2 specific primers revealed that seven of these isolates had eliminated the plasmid. Of the four colonies which were positive by PCR, one regained the ability to express a capsule upon repeated culture suggesting that the defect was reversible. This is an important observation as capsule expression is a principal marker of virulence and in the absence of PCR serves as a key diagnostic marker. The results of this preliminary study suggest that pXO2 is lost in nature and that further studies are need to determine the mechanisms by which this occurs. |
| Anahtar Kelimeler |
| Bacillus anthracis | Environmental | Plasmid stability |
| Makale Türü | Diğer (Teknik, not, yorum, vaka takdimi, editöre mektup, özet, kitap krıtiği, araştırma notu, bilirkişi raporu ve benzeri) |
| Makale Alt Türü | SCI, SSCI, AHCI, SCI-Exp dergilerinde yayımlanan teknik not, editöre mektup, tartışma, vaka takdimi ve özet türünden makale |
| Dergi Adı | Antonie van Leeuwenhoek Journal of Microbiology |
| Dergi ISSN | 0003-6072 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Makale Dili | İngilizce |
| Basım Tarihi | 09-2017 |
| Cilt No | 110 |
| Sayı | 1 |
| Sayfalar | 167 / 170 |
| Doi Numarası | 10.1007/s10482-016-0767-5 |
| Atıf Sayıları | |
| SCOPUS | 5 |
