| Yazarlar |
Fatih BÜYÜK
Kafkas Üniversitesi, Türkiye |
Mitat ŞAHİN
Kafkas Üniversitesi, Türkiye |
C Cooper
Türkiye |
Aliye GÜLMEZ SAĞLAM
Kafkas Üniversitesi, Türkiye |
|
L Baillie
Türkiye |
Dr. Öğr. Üyesi Elif ÇELİK
Kafkas Üniversitesi, Türkiye |
Doğan AKÇA
Kafkas Üniversitesi, Türkiye |
Salih OTLU
Kafkas Üniversitesi, Türkiye |
| Özet |
| The stability of the plasmid-mediated virulence factors of Bacillus anthracis, a tripartite toxin located on pXO1 and an antiphagocytic capsule encoded by genes located on pXO2, following long-term storage was investigated. A collection of 159 isolates of B. anthracis were collected from the Kars region of Turkey between 2000 and 2013 and stored at -20°C in Brucella broth supplemented with 20% glycerine. A total of 142 isolates were recovered of which one failed to express a capsule upon primary culture. A further 35 isolates yielded a mixture of mucoid and non-mucoid colonies; the majority of which had lost the pXO2 plasmid as determined by PCR analysis. Results would suggest that pXO2 is more unstable than pXO1 and that this instability increases with the length of storage. It is possible that the pXO2-deficient isolates of B. anthracis described here could be developed into a vaccine to treat at risk animals in the Kars region as many animal vaccines are based upon pXO2 deficiency. |
| Anahtar Kelimeler |
| Bacillus anthracis | pXO1 | pXO2 | Storage | Vaccine | Virulence |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | FEMS Microbiology Letters |
| Dergi ISSN | 0378-1097 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Makale Dili | İngilizce |
| Basım Tarihi | 07-2015 |
| Cilt No | 362 |
| Sayı | 13 |
| Doi Numarası | 10.1093/femsle/fnv102 |
| Atıf Sayıları | |
| SCOPUS | 10 |
