A novel approach for the waste industry: Investigating the impact of synthetic acetovanilone derivatives on thermophilic recombinant xylanase and its enzymatic effects
Yazarlar (2)
Dr. Öğr. Üyesi Orhan ULUÇAY Kafkas Üniversitesi, Türkiye
Doç. Dr. Feyzi Sinan TOKALI Kafkas Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Biomass Conversion and Biorefinery (Q2) | ||
| Dergi ISSN | 2190-6815 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 01-2024 |
| Cilt / Sayı / Sayfa | 14 / 12 / 13421–13430 | DOI | 10.1007/s13399-023-04538-6 |
| Makale Linki | https://doi.org/10.1007/s13399-023-04538-6 | ||
| Özet |
| In this study, acetovanillone derivative compounds (1–7) were synthesized, and their structures were analyzed using nuclear magnetic resonance (1H NMR—13C NMR)and Fourier-transform infrared (FTIR) methods. The xylanase gene within the scope of the study was cloned using recombinant DNA techniques. The purified recombinant protein was subjected to comprehensive characterization, including assessment of pH stability, temperature dependency, and substrate specificity. The optimal temperature and pH for the enzyme were determined to be 72 ºC and 6.0, respectively. Investigations were done into how the synthetic compounds affected the xylanase enzyme. Especially compound 2 increased the activity at concentrations of 0.1, 0.3, 0.5 and 0.7 mM by 176.9, 153.25, 146.22 and 115.41%, respectively, compared to the positive control. It has also been observed that the effect on activity decreases with … |
| Anahtar Kelimeler |
| Acetovanillone | Cloning | Synthesis | Waste industry | Xylanase |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Google Scholar | 1 |
| Web of Science | 1 |
| Scopus | 1 |