| Yazarlar |
Dr. Öğr. Üyesi Orhan ULUÇAY
Kafkas Üniversitesi, Türkiye |
Doç. Dr. Feyzi Sinan TOKALI
Kafkas Üniversitesi, Türkiye |
| Özet |
| In this study, acetovanillone derivative compounds (1–7) were synthesized, and their structures were analyzed using nuclear magnetic resonance (1H NMR—13C NMR)and Fourier-transform infrared (FTIR) methods. The xylanase gene within the scope of the study was cloned using recombinant DNA techniques. The purified recombinant protein was subjected to comprehensive characterization, including assessment of pH stability, temperature dependency, and substrate specificity. The optimal temperature and pH for the enzyme were determined to be 72 ºC and 6.0, respectively. Investigations were done into how the synthetic compounds affected the xylanase enzyme. Especially compound 2 increased the activity at concentrations of 0.1, 0.3, 0.5 and 0.7 mM by 176.9, 153.25, 146.22 and 115.41%, respectively, compared to the positive control. It has also been observed that the effect on activity decreases with increasing concentration. Compound 3, on the contrary, increased the activity at high concentrations (0.7 and 1 mM) by 147.30 and 153.93%, respectively. Considering the positive sample (100%), CaCI2 shows 70.38% activity at 0.1 mM, 68.04% at 0.3 mM, 79.96% at 0.5 mM, 61.99% at 0.7 mM, and 85.59% at 1 mM.These results show that CaCI2 causes a slight decrease in enzyme activity.ZnCI2, the zinc compound, and CuCI2, the copper compound, showed a similar decrease in activity. It was determined that these different reactions showed different relative activity of the metal ions at varying concentrations for the recombinant xylanase enzyme. |
| Anahtar Kelimeler |
| Acetovanillone | Cloning | Synthesis | Waste industry | Xylanase |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | Springer Science and Business Media LLC |
| Dergi ISSN | 2190-6815 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q2 |
| Makale Dili | Türkçe |
| Basım Tarihi | 01-2023 |
| Doi Numarası | 10.1007/s13399-023-04538-6 |
| Makale Linki | http://dx.doi.org/10.1007/s13399-023-04538-6 |
| Atıf Sayıları |
