| Yazarlar |
Emin Karakurt
Faculty of Veterinary Medicine, Kars |
|
Nuvit Coskun
Faculty of Veterinary Medicine, Kars |
|
Enver Beytut
Faculty of Veterinary Medicine, Kars |
|
Serpil Dag
Faculty of Veterinary Medicine, Kars |
|
Volkan Yilmaz
Faculty of Veterinary Medicine, Kars |
|
Hilmi Nuhoglu
Faculty of Veterinary Medicine, Kars |
|
Ayfer Yildiz
Faculty of Veterinary Medicine, Kars |
|
Emre Kurtbas
Kafkas University, Turkey. |
| Özet |
| In this study, in order to reveal the immune response against the disease in naturally infected sheep with SPPV, the expressions of various pro- or anti-inflammatory cytokines such as tumour necrosis factor alpha (TNF-α), interferon gamma (IFN-γ), interleukin-1beta (IL-1β), interleukin-2 (IL-2), interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-10 (IL-10) and interleukin-12 (IL-12) were evaluated immunohistochemically. The material of this study consisted of tissue samples taken from 24 sheep, which were brought as dead for routine histopathological examination to the Department of Pathology. Avidin–biotin–peroxidase method was used for immunohistochemistry. Characteristic pox lesions were observed in the skin, lungs and kidneys. In histopathological examinations, pox cells, which are very characteristic for the diagnosis of the disease, were observed in all three tissues. Capripoxvirus nucleic acid was detected in 8 of the 24 tissues. Samples were sequenced, and a phylogenetic tree was constructed with reference strains from GenBank. Strains from the study clustered with sheeppox virus references. In conclusion, the levels of pro-inflammatory cytokines such as TNF-α, IFN-γ, IL-1β, IL-2, IL-8 and IL12 (Th1) were much more dominant compared to the levels of anti-inflammatory cytokines: IL-10 and IL-6 (Th2). This supported the fact that the cellular immune response is much more effective than the humoral immune response in sheeppox. |
| Anahtar Kelimeler |
| Cytokine | Immune response | Lamb | Polymerase chain reaction | Sheeppox virus |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | Tropical animal health and production |
| Dergi ISSN | 1573-7438 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q2 |
| Makale Dili | İngilizce |
| Basım Tarihi | 12-2023 |
| Cilt No | 55 |
| Sayı | 6 |
| Sayfalar | 401 / |
| Doi Numarası | 10.1007/s11250-023-03823-w |
| Makale Linki | https://link.springer.com/article/10.1007/s11250-023-03823-w |
| Atıf Sayıları |
