| Yazarlar |
Sevda ELİŞ YILDIZ
Türkiye |
Arzu Gezer
Türkiye |
Ebru KARADAĞ SARI
Türkiye |
| Özet |
| OBJECTIVE This experimental study was aimed to assess the positive or negative effects of the radioprotective agent amifostine (AMF) (WR-2721) on mucin 1 (MUC1) through immunohistochemical methods in pre- venting the damage that may occur in the prostate tissue after radiotherapy (RT). METHODS In the experimental period, 32 Sprague–Dawley male rats were divided into 4 groups: Untreated control group, AMF group; 200 mg AMF was intraperitoneally administered to the rats in the second group for 3 days. The rats in the third group (RT-saline) were experimentally administered a total of 6 Gy includ- ing 2 Gy of RT in 3 fractions. The rats in the fourth group (RT – AMF) received totally 6 Gy RT and 200 mg/kg AMF for 3 days. The rats were sacrificed for histological assessments 4 weeks after RT. Triple staining was performed to the tissues for histopathological examinations and immunohistochemical localization of MUC1 in prostate tissue was determined using streptavidin-biotin peroxidase method. RESULTS Histopathologically, it was observed that the parenchymal and stromal cells of the prostate tissues in all groups had a structurally normal appearance. Immunohistochemically, the MUC1 immunoreactivity was weak in the parenchymal and stromal cells of the control group, and strong MUC1 secretion was observed in the AMF group, AMF RT group, and RT group. CONCLUSION When considering the effects of AMF application on MUC1 secretion in the prostate tissue of rats treated with RT, it is thought that the protective effect of AMF may vary in tissues, depending on the dose applied and the number of fractions. |
| Anahtar Kelimeler |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | ESCI dergilerinde yayımlanan tam makale |
| Dergi Adı | TURKISH JOURNAL of ONCOLOGY |
| Dergi ISSN | 1300-7467 |
| Dergi Tarandığı Indeksler | TR DİZİN |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2023 |
| Sayı | 3 |
| Sayfalar | 307 / 313 |
| Doi Numarası | 10.5505/tjo.2023.3974 |
| Makale Linki | http://dx.doi.org/10.5505/tjo.2023.3974 |
| Atıf Sayıları |
