Reusable nanocopy machine particles for the replication of DNA
Yazarlar (5)
Rıdvan Say
Anadolu Üniversitesi, Türkiye
Anadolu Üniversitesi, Türkiye
Özlem Ünlüer Anadolu Üniversitesi, Türkiye
Arzu Ersöz Anadolu Üniversitesi, Türkiye
Dr. Öğr. Üyesi Cem ÖZİÇ Kafkas Üniversitesi, Türkiye
Volkan Kılıç Anadolu Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Biotechnology Progress | ||
| Dergi ISSN | 8756-7938 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI | ||
| Makale Dili | İngilizce | Basım Tarihi | 02-2015 |
| Cilt / Sayı / Sayfa | 31 / 1 / 119–123 | DOI | 10.1002/btpr.2016 |
| Makale Linki | http://doi.wiley.com/10.1002/btpr.2016 | ||
| Özet |
| As one of the most important components copying DNA molecules in the PCR system, Taq DNA polymerase has a high processivity, however, lower persistence when compared to other polymerases. Studies for the enhancement of stability of Taq DNA polymerase is of great importance. The present study describes the integration of PCR application of cross‐linked Taq DNA polymerase enzyme in a nanochamber using a ruthenium based MATyr‐Ru‐(bipyr)2)‐MATyr monomer hapten prepared by photosensitive microemulsion polymerization technique. The conjugation and cross‐linking have achieved using our previously invented Aminoacid (monomer) Decorated and Light Underpining Conjugation Approach (ANADOLUCA) method. Microemulsion polymerization media has prepared by dispersing PVA in deionized water. The nano enzyme could be easily prepared at room temperature, in daylight and under nitrogen atmosphere using ruthenium based photosensitive cross‐linking agents. The nano copy machine particles (nano Taq DNA polymerase) are very stable against more acidic or more basic conditions, high temperatures and could be reusable in PCR analysis for many times without any deformation in their structures. © 2014 American Institute of Chemical Engineers Biotechnol. Prog., 31:119–123, 2015 |
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