| Yazarlar |
Pınar BAYRAM
Kafkas Üniversitesi, Türkiye |
Selina AKSAK KARAMEŞE
Kafkas Üniversitesi, Türkiye |
Bengül Özdemir
Türkiye |
|
Çağatay Salum
Kastamonu Üniversitesi, Türkiye |
|
Hüseyin Serkan Erol
Kastamonu Üniversitesi, Türkiye |
Murat KARAMEŞE
Türkiye |
| Özet |
| In this study, our aim was to investigate the possible protective and therapeutic effects of these two flavonoids, baicalein, and naringin, in 50 and 100 mg/kg doses applied both before and after cecal ligation and puncture (CLP) procedures in a polymicrobial sepsis rat model, and evaluate the possible contribution of oxidative and inflammatory markers by immunological, biochemical, molecular, and histopathological methods. Sixty-six Wistar albino rats were divided into 11 groups. The pro-inflammatory (TNF-alpha, IL-1-beta, and IL-6) and anti-inflammatory (TGF-beta and IL-10) cytokine levels were measured by ELISA technique. CD3, CD68, and nuclear factor kappa B positivity rates were detected by immunohistochemical methods. Oxidative stress parameters (MDA, SOD, and GSH) were measured by tissue biochemistry. Sepsis caused a significant increase in all pro-inflammatory cytokine levels and MDA activity. Also, it led to an increase in the positivities of CD3, CD68, and NF-κB markers. However, especially pre-CLP doses of baicalein and naringin inhibited the inflammation process by suppressing pro-inflammatory and increasing anti-inflammatory cytokine levels, as well as regulating the oxidative stress process by normalizing the oxidant/anti-oxidant enzyme levels. Both pre- and post-application of baicalein and naringin are quite effective to prevent sepsis-caused cellular processes. This protective and therapeutic effects by baicalein and naringin in animals with sepsis seems to be originated from the high antioxidant capacity and inhibition of pro-inflammatory cytokine production. Thus, those natural agents may prove to be valuable protective agent against septic shock. |
| Anahtar Kelimeler |
| Sepsis | flavonoids | baicalein | naringin | inflammation | oxidative stress | CLP | TNF-alpha | IL-6 |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY |
| Dergi ISSN | 0892-3973 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q2 |
| Makale Dili | Türkçe |
| Basım Tarihi | 09-2023 |
| Cilt No | 45 |
| Sayı | 5 |
| Sayfalar | 597 / 606 |
| Doi Numarası | 10.1080/08923973.2023.2197143 |
| Makale Linki | http://dx.doi.org/10.1080/08923973.2023.2197143 |
| Atıf Sayıları | |
| WoS | 8 |
