Immobilization with Ca–Alg@gelatin hydrogel beads enhances the activity and stability of recombinant thermoalkalophilic lipase
Yazarlar (2)
Doç. Dr. Kezban YILDIZ DALGINLI Kafkas Üniversitesi, Türkiye
Prof. Dr. Onur ATAKİŞİ Kafkas Üniversitesi, Türkiye
| Makale Türü |
Özgün Makale
(SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
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| Dergi Adı | CHEMICAL AND PROCESS ENGINEERING-NEW FRONTIERS (Q4) | ||
| Dergi ISSN | 0208-6425 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 01-2023 |
| Cilt / Sayı / Sayfa | 44 / 1 / 1–16 | DOI | 10.24425/cpe.2023.142291 |
| UAK Araştırma Alanları |
Biyokimya
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| Özet |
| This study aims at the immobilization and characterization of thermoalkalophilic lipases produced recombinantly from Bacillus thermocatenulatus BTL2 and Bacillus pumilus MBB03. For this purpose, immobilization of the produced enzymes in calcium-alginate@ gelatin (Ca–Alg@ gelatin) hydrogel beads, immobilization optimization and characterization measurements of the immobilized-enzyme hydrogels were conducted. Optimum temperature and pH values were determined for B. thermocatenulatus and B. pumilus MBB03 immobilized-enzyme hydrogels (60–70◦ C, 55◦ C and pH 9.5, pH 8.5). Thermal stability was determined between 65◦ C and 60◦ C of B. thermocatenulatus and B. pumilus MBB03 immobilized enzymes, respectively. The pH stability was determined between pH 7.0–11.0 at+ 4◦ C and pH 8.0–10.0 at+ 4◦ C, respectively.In conclusion, the entrapment technique provided controlled production of small diameter hydrogel beads (∼ 0: 19 and∼ 0: 29) with negligible loss of enzyme. These beads retained high lipase activity at high pH and temperature. The activity of Ca–Alg@ gelatin-immobilized lipase remained relatively stable for up to three cycles and then markedly decreased. With this enzyme immobilization, it may have a potential for use in esterification and transesterification reactions carried out in organic solvent environments. We can conclude that it is one of the most promising techniques for highly efficient and economically competent biotechnological processes in the field of biotransformation, diagnostics, pharmaceutical, food and detergent industries. |
| Anahtar Kelimeler |
| alginate | gelatin | hydrogel beads | thermoalkalophilic lipase | immobilization | characterization |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 6 |
| Google Scholar | 6 |