| Yazarlar |
Prof. Dr. İnan KAYA
Kafkas Üniversitesi, Türkiye |
Prof. Dr. Serpil DAĞ
Türkiye |
Dr. Öğr. Üyesi Müge MAVİOĞLU KAYA
Kafkas Üniversitesi, Türkiye |
Erdi Anıl Tanrıverdi
Türkiye |
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Hatice Beşeren
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Gizem Aşasın
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| Özet |
| Objectives: This study aimed to evaluate the effects of pomegranate fruit extract (PFE) on levels of transient receptor potential (TRP) channel and caspase-3 (Casp-3) expressions, tumor necrosis factor-α (TNF-α), total sialic acid (TSA), reduced glutathione (GSH), and malondialdehyde (MDA) in mice with induced colorectal cancer (CRC) by investigating effects of PFE on in vitro mitotic index (MI). Methods: Different PFE concentrations on MI against 0.3 μg/mL mitomycin-C (MMC) in cell culture were evaluated by binocular light microscopy. During in vivo applications on Balb/c mice, it was given once physiological saline to group I, PFE for ten weeks to group II, a single dose of azoxymethane (AOM) plus dextran sulfate sodium in drinking water (DSS) to group III, and AOM plus DSS plus PFE to group IV. Tissue samples were evaluated by western blotting, spectrophotometric, and histopathological methods. Results: Expressions of Casp-3, TRP ankyrin 1 (TRPA1), and melastatin 2 (TRPM2) channels and TNF-α, TSA, GSH, and MDA concentrations in evaluated tissues had significantly better levels in PFE-treated groups compared to CRC-induced mice. Conclusions: Results of the present study indicate that PFE application in mice with induced CRC may be an important modulator of TRPA1 and TRPM2 channels, apoptosis, and inflammatory response by decreasing oxidative stress. |
| Anahtar Kelimeler |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | Turkish Journal of Biochemistry |
| Dergi ISSN | 1303-829X |
| Dergi Tarandığı Indeksler | SCI |
| Dergi Grubu | Q4 |
| Makale Dili | Türkçe |
| Basım Tarihi | 08-2022 |
| Cilt No | 47 |
| Sayı | 5 |
| Doi Numarası | 10.1515/tjb-2022-0099 |
| Makale Linki | http://dx.doi.org/10.1515/tjb-2022-0099 |
| Atıf Sayıları |
