Modulatory effect of pomegranate extract on TRPA1, TRPM2 and caspase-3 expressions in colorectal cancer induction of mice
Yazarlar (6)
Prof. Dr. İnan KAYA
Kafkas Üniversitesi, Türkiye
Prof. Dr. Serpil DAĞ
Kafkas Üniversitesi, Türkiye
Dr. Öğr. Üyesi Müge MAVİOĞLU KAYA
Kafkas Üniversitesi, Türkiye
Erdi Anıl Tanrıverdi
Kafkas Üniversitesi, Türkiye
Hatice Beşeren
Kafkas Üniversitesi, Türkiye
Gizem Aşasın
Kafkas Üniversitesi, Türkiye
| Makale Türü |
Özgün Makale
(SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
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| Dergi Adı | Türk Biyokimya Dergisi (Q4) | ||
| Dergi ISSN | 1303-829X Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI | ||
| Makale Dili | İngilizce | Basım Tarihi | 08-2022 |
| Cilt / Sayı / Sayfa | 47 / 5 / 612–619 | DOI | 10.1515/tjb-2022-0099 |
| Makale Linki | http://dx.doi.org/10.1515/tjb-2022-0099 | ||
| Özet |
| Objectives: This study aimed to evaluate the effects of pomegranate fruit extract (PFE) on levels of transient receptor potential (TRP) channel and caspase-3 (Casp-3) expressions, tumor necrosis factor-α (TNF-α), total sialic acid (TSA), reduced glutathione (GSH), and malondialdehyde (MDA) in mice with induced colorectal cancer (CRC) by investigating effects of PFE on in vitro mitotic index (MI). Methods: Different PFE concentrations on MI against 0.3 μg/mL mitomycin-C (MMC) in cell culture were evaluated by binocular light microscopy. During in vivo applications on Balb/c mice, it was given once physiological saline to group I, PFE for ten weeks to group II, a single dose of azoxymethane (AOM) plus dextran sulfate sodium in drinking water (DSS) to group III, and AOM plus DSS plus PFE to group IV. Tissue samples were evaluated by western blotting, spectrophotometric, and histopathological methods. Results: Expressions of Casp-3, TRP ankyrin 1 (TRPA1), and melastatin 2 (TRPM2) channels and TNF-α, TSA, GSH, and MDA concentrations in evaluated tissues had significantly better levels in PFE-treated groups compared to CRC-induced mice. Conclusions: Results of the present study indicate that PFE application in mice with induced CRC may be an important modulator of TRPA1 and TRPM2 channels, apoptosis, and inflammatory response by decreasing oxidative stress. |
| Anahtar Kelimeler |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları |