| Yazarlar |
Tolga Sungur
Gazi Üniversitesi, Turkey |
|
Sirri Kar
Gazi Üniversitesi, Turkey |
|
Esin Güven
Gazi Üniversitesi, Turkey |
|
Metin Aktaş
Gazi Üniversitesi, Turkey |
|
Zafer Karaer
Gazi Üniversitesi, Turkey |
|
Zati Vatansever
Gazi Üniversitesi, Turkey |
| Özet |
| This study was carried out on 45 stool specimens, consisting of 18 samples from children with diarrhea and 27 samples from diarrheic calves. Samples were screened by both carbol fuchsin staining and nested PCR for presence of Cryptosporidium spp. Using the carbol fuchsin staining method, we detected a total of 4 (8.9%) positive samples out of 45; of these 3 (11.2%) were from calf samples and 1 (5.6%) from a child. Nested PCR detected a total of 9 (20.0% positive samples out of 45 including 8 (29.7%) from calf samples and 1 (5.6%) from a child. Although the staining method revealed a 100% specificity, it was deficient in sensitivity (44.0%) compared to nested PCR. The study showed that nested PCR is an acceptable method for studying the etiology of doubtful diarrheal cases. |
| Anahtar Kelimeler |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SCOPUS dergilerinde yayımlanan tam makale |
| Dergi Adı | Türkiye parazitolojii dergisi / Türkiye Parazitoloji Derneǧi = Acta parasitologica Turcica / Turkish Society for Parasitology |
| Dergi ISSN | 1300-6320 |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2008 |
| Cilt No | 32 |
| Sayı | 4 |
| Sayfalar | 305 / 308 |
