Detection of Cryptosporidium spp. in feces with nested PCR and carbol fuchsin staining method
Yazarlar (6)
Tolga Sungur
Gazi Üniversitesi, Türkiye
Sirri Kar
Gazi Üniversitesi, Türkiye
Esin Güven
Gazi Üniversitesi, Türkiye
Metin Aktaş
Gazi Üniversitesi, Türkiye
Zafer Karaer
Gazi Üniversitesi, Türkiye
Prof. Dr. Zati VATANSEVER
Kafkas Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SCOPUS dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Turkiye Parazitolojii Dergisi Turkiye Parazitoloji Dernei Acta Parasitologica Turcica Turkish Society for Parasitology | ||
| Dergi ISSN | 1300-6320 Scopus Dergi | ||
| Makale Dili | İngilizce | Basım Tarihi | 01-2008 |
| Cilt / Sayı / Sayfa | 32 / 4 / 305–308 | DOI | – |
| Özet |
| This study was carried out on 45 stool specimens, consisting of 18 samples from children with diarrhea and 27 samples from diarrheic calves. Samples were screened by both carbol fuchsin staining and nested PCR for presence of Cryptosporidium spp. Using the carbol fuchsin staining method, we detected a total of 4 (8.9%) positive samples out of 45; of these 3 (11.2%) were from calf samples and 1 (5.6%) from a child. Nested PCR detected a total of 9 (20.0% positive samples out of 45 including 8 (29.7%) from calf samples and 1 (5.6%) from a child. Although the staining method revealed a 100% specificity, it was deficient in sensitivity (44.0%) compared to nested PCR. The study showed that nested PCR is an acceptable method for studying the etiology of doubtful diarrheal cases. |
| Anahtar Kelimeler |
