Detection of Cryptosporidium spp. in feces with nested PCR and carbol fuchsin staining method
Yazarlar (6)
Tolga Sungur
Gazi Üniversitesi, Türkiye
Gazi Üniversitesi, Türkiye
Sirri Kar Gazi Üniversitesi, Türkiye
Esin Güven Gazi Üniversitesi, Türkiye
Metin Aktaş Gazi Üniversitesi, Türkiye
Zafer Karaer
Gazi Üniversitesi, Türkiye
Gazi Üniversitesi, Türkiye
Prof. Dr. Zati VATANSEVER Kafkas Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SCOPUS dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Turkiye Parazitolojii Dergisi Turkiye Parazitoloji Dernei Acta Parasitologica Turcica Turkish Society for Parasitology | ||
| Dergi ISSN | 1300-6320 Scopus Dergi | ||
| Makale Dili | İngilizce | Basım Tarihi | 01-2008 |
| Cilt / Sayı / Sayfa | 32 / 4 / 305–308 | DOI | 10.5555/20093034175 |
| Makale Linki | https://www.cabidigitallibrary.org/doi/full/10.5555/20093034175 | ||
| Özet |
| 45 faecal specimens, including 18 from children and 27 from calves with diarrhoea [in Turkey], were screened for Cryptosporidium spp. using carbol fuchsin staining and nested polymerase chain reaction (PCR). The carbol fuchsin staining method detected 4 (8.9%) positive samples, including 3 (11.2%) from calves and 1 (5.6%) from a child. The nested PCR detected 9 (20.0%) positive samples, including 8 (29.7%) from calves and 1 (5.6%) from a child. Although the carbol fuchsin staining method revealed 100% specificity, it was deficient in sensitivity (44.0%) as compared to the nested PCR. It is concluded that the nested PCR is an acceptable method for studying the aetiology of doubtful diarrhoeal cases. |
| Anahtar Kelimeler |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Scopus | 7 |