Yazarlar |
Prof. Dr. Özgür ÇELEBİ
Kafkas Üniversitesi, Türkiye |
Tolga Bahadır
|
İsmail Şimşek
|
Furkan Aydın
|
Halil İbrahim Kahve
|
Şevket Tulun
|
Prof. Dr. Fatih BÜYÜK
Türkiye |
Hakan Celebi
|
Özet |
The changes in the surface chemistry and morphological structure of chitin forms obtained from shrimp shells (Shp[sbnd]S) with and without microorganisms were evaluated. Total mesophilic aerobic bacteria (TMAB), estimated Pseudomonas spp. and Enterococcus spp. were counted in Shp-S by classical cultural counting on agar medium, where the counts were 6.56 ± 0.09, 6.30 ± 0.12, and 3.15 ± 0.03 CFU/g, respectively. Fourier Transform Infrared (FTIR) Spectroscopy and Scanning Electron Microscopy (SEM)/Energy dispersed X-ray (EDX) were used to assess the surface chemistry/functional groups and morphological structure for ChTfree (non-microorganism), and ChTmo (with microorganisms). ChTfree FTIR spectra presented a detailed chitin structure by O[sbnd]H, N[sbnd]H, and C[sbnd]O stretching vibrations, whereas specific peaks of chitin could not be detected in ChTmo. Major differences were also found in SEM analysis for ChTfree and ChTmo. ChTfree had a flat, prominent micropore, partially homogeneous structure, while ChTmo had a layered, heterogeneous, complex dense fibrous, and lost pores form. The degree of deacetylation was calculated for ChTfree and ChTmo according to FTIR and EDX data. The results suggest that the degree of deacetylation decreases in the presence of microorganisms, affecting the production of beneficial components negatively. The findings were also supported by the molecular docking model. |
Anahtar Kelimeler |
Chitin | Docking | FTIR | Microbiological analyzes | SEM/EDX | Shrimp shell |
Makale Türü | Özgün Makale |
Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
Dergi Adı | International Journal of Biological Macromolecules |
Dergi ISSN | 0141-8130 |
Dergi Tarandığı Indeksler | SCI-Expanded |
Dergi Grubu | Q1 |
Makale Dili | İngilizce |
Basım Tarihi | 02-2024 |
Cilt No | 263 |
Sayı | 1 |
Sayfalar | 130353 / 0 |
Doi Numarası | 10.1016/j.ijbiomac.2024.130353 |
Makale Linki | https://www.sciencedirect.com/science/article/pii/S0141813024011565?via%3Dihub |