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A phylogenetic analysis of Mycoplasma strains circulating in sheep pneumonia in the Kars region of Turkey    
Yazarlar
Prof. Dr. Salih OTLU Prof. Dr. Salih OTLU
Türkiye
Dr. Öğr. Üyesi Olcay ÖZTÜRKLER Dr. Öğr. Üyesi Olcay ÖZTÜRKLER
Kafkas Üniversitesi, Türkiye
Özet
This study aimed to investigate Mycoplasma species by cultural and molecular methods in 250 pneumonics and 30 healthy
appearance sheep lungs brought from slaughterhouse and butcheries in the Kars region of Turkey. The phylogenetic positioning of
the bacterial strains to be obtained in the present study was another remarkable outcome of the study. Mycoplasma isolation was
achieved solely in 26 (10.4%) of 250 sheep lungs with pneumonia. Mycoplasma ovipneumoniae was found predominant species in 12
(46.15%) of samples and that was followed by Mycoplasma arginini in 4 (15.38%) of strains. Moreover, two samples were found to be
co-infected with the aforementioned Mycoplasma species and 8 were found negative. The partial sequence analysis of the 16S rRNA
gene of 22 (84.61%) isolates emerged that 11 of the isolates were M. ovipneumoniae and 4 were M. arginini. Two mixed samples were
further identified as M. arginini by the sequence analysis. Five isolates that were not identified by cultural and PCR procedures were
identified as M. bovigenitalium by sequence analysis. The evolutionary analysis of the 16S rRNA sequence yielded three main clusters
as M. ovipneumoniae, M. arginini, and M. bovigenitalium which took part in the Hominis group that was previously described in
Mycoplasma phylum. The inter se proximity of the main clusters was thus described polyphyletic for M. ovipneumoniae and M. arginini
and monophyletic for M. bovigenitalium. However, in the general view, M. arginini and M. bovigenitalium isolates had two distinct
phylogenetic positions, but their proximity to each other was higher than those obtained for M. ovipneumoniae isolates.
Anahtar Kelimeler
Mycoplasma | sheep | pneumonia | 16S rRNA | sequence analysis | phylogeny
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES
Dergi ISSN 1300-0128
Dergi Tarandığı Indeksler SCI-Expanded
Dergi Grubu Q4
Makale Dili Türkçe
Basım Tarihi 01-2020
Cilt No 44
Sayı 4
Sayfalar 805 / 813
Doi Numarası 10.3906/vet-2001-59
Makale Linki http://dx.doi.org/10.3906/vet-2001-59