Assessing the impact of dental restorative materials on fibroblast cells: an immunohistochemical and ELISA analysis
Yazarlar (4)
Dr. Öğr. Üyesi Mustafa Düzyol İstanbul Medeniyet Üniversitesi, Türkiye
Doç. Dr. Pınar BAYRAM Kafkas Üniversitesi, Türkiye
Dr. Öğr. Üyesi Esra Düzyol İstanbul Medeniyet Üniversitesi, Türkiye
Prof. Dr. Selina AKSAK KARAMEŞE Kafkas Üniversitesi, Türkiye
| Makale Türü |
Özgün Makale
(SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
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| Dergi Adı | Scientific Reports (Q1) | ||
| Dergi ISSN | 2045-2322 Dergi Bilgileri (2024) | ||
| Dergi Tarandığı Indeksler | SCI | ||
| Makale Dili | Türkçe | Basım Tarihi | 02-2024 |
| Kabul Tarihi | 12-02-2024 | Yayınlanma Tarihi | 27-02-2024 |
| Cilt / Sayı / Sayfa | 14 / 0 / – | DOI | 10.1038/s41598-024-54331-2 |
| Makale Linki | http://dx.doi.org/10.1038/s41598-024-54331-2 | ||
| UAK Araştırma Alanları |
Histoloji ve Embriyoloji
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| Özet |
| In this study, our aim was to investigate the effects of restorative materials such as composite, compomer and high viscosity glass ionomer, which are frequently used in dentistry, on L929 fibroblast cells by evaluating the oxidative stress parameters, pro- and anti-inflammatory cytokines, and apoptosis markers. L929 fibroblast cells were cultured, and dental filling materials were applied in two doses (50 and 100 µl). Immunohistochemical staining was performed for experimental groups with Anti-Bax and Anti-Caspase 9 antibodies. Then, ELISA technique was used to detect the level of TNF-alpha, TGF-beta, IL-1-beta, IL-6, IL-10, LPO and CAT. In the light of the data, the examined dental filling materials were effective on increasing the TGF-beta, IL-10, LPO and CAT levels, and decreasing the TNF-alpha, IL-1-beta, and IL-6 levels. The histological micrographs were also support the issues. When the levels of H-score in Caspase 9 labeled micrographs were evaluated, the mean of the control group was lower than the mean of the experimental groups. Biocompatibility varies according to the content of the material, the amount of residual monomer, and its solubility. Although all the experimental groups have cytotoxic effects, the least effect is seen in the Omnichroma group. |
| Anahtar Kelimeler |
| Apoptosis | Dental filling materials | Fibroblast | Inflammation | Oxidative stress |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Yöksis | 4 |
| PubMed | 3 |