Plasma Nitric Oxide (NO) and Tumor Necrosis Factor-α (TNF-α) levels, Adenosine Deaminase (ADA), Gamma Glutamyl Transferase (GGT) activities and to determine the rate of lymphocytes in the peripheral blood leukocytes Alpha Naphthyl Acetate Esterase (ANAE) in cattle with leptospirosis
Yazarlar (10)
Emine Atakişi Kafkas Üniversitesi, Türkiye
Ali Haydar Kirmizigül Kafkas Üniversitesi, Türkiye
Onur Atakişi Kafkas Üniversitesi, Türkiye
Ebru Karadaǧ Sari Kafkas Üniversitesi, Türkiye
Metin Öǧün Kafkas Üniversitesi, Türkiye
Şaban Maraşli Kafkas Üniversitesi, Türkiye
Ayla Özcan Kafkas Üniversitesi, Türkiye
Mahmut Karapehlivan Kafkas Üniversitesi, Türkiye
Prof. Dr. Fatih BÜYÜK Kafkas Üniversitesi, Türkiye
Özgür Çelebi Kafkas Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SCOPUS dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Kafkas Universitesi Veteriner Fakultesi Dergisi | ||
| Dergi ISSN | 1300-6045 Wos Dergi Scopus Dergi | ||
| Makale Dili | İngilizce | Basım Tarihi | 05-2014 |
| Cilt / Sayı / Sayfa | 20 / 3 / 451–455 | DOI | 10.9775/kvfd.2013.10427 |
| Makale Linki | https://doaj.org/article/4a05b56f9b23405095f6311ad5d1f84f | ||
| Özet |
| In this study, by determining in plasma the nitric oxide (NO), tumour necrosis factor-α (TNF-α) levels, adenosine deaminase (ADA), gamma glutamyl transferase (GGT) activities and percentage of T lymphocytes with alpha naphthyl acetate esterase method (ANAE), it was aimed to investigate the immunopathogenesis and biochemical mechanism of bovine leptospirosis. Blood samples of twenty healthy calves and twenty calves with leptospirosis which grown in the villages around Kars were collected into heparinised tubes from jugular vein. After taking the blood smear, blood samples were centrifuged at 3.000 rpm for 15 min. Plasma NO levels and ADA activities were determined with chemical method. TNF-α levels and GGT activities were determined with commercial kits. T/B lymphocyte ratios were determined at peripheral blood leukocytes with ANAE method. Plasma TNF-α and NO levels, ADA and GGT activities in animals with leptospirosis were found to be high P<0.001, P<0.05, P<0.05, P<0.05 respectively compared to healthy animals. ANAE positive T lymphocytes ratio were determined as 43%. As a result, TNF-α as a cytokine can be determined in early stage of infection and, accordingly, significantly increased levels of NO, ADA and GGT activities and, on the basis of the increased in the percentage of B lymphocytes, it is proposed to these parameters could be used for determining in explain the pathogenesis of the disease. It is tought that, it must be done more studies on biochemical mechanism of leptospirosis besides native infected cattle. |
| Anahtar Kelimeler |
| ADA | Calf | GGT | Leptospirosis | NO | TNF-α |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| WoS | 2 |
| SCOPUS | 2 |