| Yazarlar |
Erdal Horata
Türkiye |
|
Hakan Ay
Eskişehir Osmangazi Üniversitesi, Türkiye |
Dr. Öğr. Üyesi Duygu ASLAN
Türkiye |
| Özet |
| The contribution of the thalamus to the development and behavioural changes in autism spectrum disorders (ASD), a neurodevelopmental syndrome, remains unclear. The aim of this study was to determine the changes in thalamic volume and cell number in the valproic acid (VPA)-induced ASD model using stereological methods and to clarify the relationship between thalamus and ASD-like behaviour. Ten pregnant rats were administered a single dose (600 mg/kg) of VPA intraperitoneally on G12.5 (VPA group), while five pregnant rats were injected with 5 ml saline (control group). Behavioural tests were performed to determine appropriate subjects and ASD-like behaviours. At P55, the brains of the subjects were removed. The sagittal sections were stained with cresyl violet and toluidine blue. The thalamic and hemispheric volumes with their ratios, the total number of thalamic cells, neurons and non-neuronal cells were calculated using stereological methods. Data were compared using a t-test and a Pearson correlation analysis was performed to examine the relationship between behaviour and stereological outcomes. VPA-treated rats had lower sociability and sociability indexes. There was no difference in social novelty preference and anxiety. The VPA group had larger hemispheric volume, lower thalamic volume, and fewer neurons. The highest percentage decrease was in non-neuronal cells. There was a moderate positive correlation between the number of non-neuronal cells and sociability, thalamic volume and the number of neurons as well as the time spent in the light box. The correlation between behaviour and stereological data suggests that the thalamus is associated with ASD-like behaviour. |
| Anahtar Kelimeler |
| Autism spectrum disorder | Valproic acid | Three-chamber social interaction | Light/dark transition | Thalamus | Stereology |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | BRAIN RESEARCH |
| Dergi ISSN | 0006-8993 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q3 |
| Makale Dili | Türkçe |
| Basım Tarihi | 10-2024 |
| Cilt No | 1840 |
| Sayfalar | 149047 / |
| Doi Numarası | 10.1016/j.brainres.2024.149047 |
| Makale Linki | http://dx.doi.org/10.1016/j.brainres.2024.149047 |
| Atıf Sayıları |
