| Yazarlar |
| Özet |
| BACKGROUND Quantification of nitisinone, 2-(nitro-4-trifluoromethylbenzoyl)1,3-cyclohexanedione (NTBC) has been repeatedly described. Nevertheless monitoring of NTBC has not yet become part of routine therapy surveillance in tyrosinaemia type I (OMIM 276700). We developed a blood spot test to facilitate collection and transport of samples. Furthermore, the test material can be used for determination of other parameters like tyrosine and succinylacetone. METHOD For quantification of NTBC in blood spots filter paper discs of 3.2mm diameter were extracted with 150μL methanol containing mesotrione as internal standard (IS). Analysis was done by UPLC--MS/MS on a Xevo mass spectrometer (ESI+), (MRM). Parent ions were 330.05 for NTBC and 340.05 for IS, daughter ions were m/z 217.95 and m/z 125.95 for NTBC, and m/z 227.95 and m/z 103.95 for IS. RESULTS The calibration curve for NTBC in blood ... |
| Anahtar Kelimeler |
| Blood spot testing | Monitoring therapy | Nitisinone | NTBC | Tyrosinaemia type I | Tyrosine |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | Clinica Chimica Acta |
| Dergi ISSN | 1873-3492 |
| Dergi Tarandığı Indeksler | |
| Dergi Grubu | Q2 |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2011 |
| Cilt No | 412 |
| Sayı | 1 |
| Sayfalar | 134 / 138 |
| Doi Numarası | 10.1016/j.cca.2010.09.027 |
| Atıf Sayıları | |
| SCOPUS | 25 |
