| Yazarlar |
Dr. Öğr. Üyesi Cem ÖZİÇ
Türkiye |
Barış Yıldız
Türkiye |
|
Ramazan Demirel
|
Prof. Dr. Özkan ÖZDEN
Türkiye |
| Özet |
| Zinc oxide nanoparticles (ZnO-NPs) are metal oxide NPs that have high cytotoxicity on cancer cells and low cytotoxicity on healthy cells. Breast cancer is the most frequent type of cancer-causing death among women worldwide. In this study, anti-cancer effects of ZnO-NPs were investigated. For this purpose, we treated the MCF7 and MDA-MB-231 breast cancer cell lines and human umbilical vein endothelial cells HUVEC cell line with 10 μg/mL and 20 μg/mL ZnO-NP. Anti-cancer effects of ZnO-NPs were evaluated with cell viability, apoptotic index and colony formation assays, and anti-Warburg effect were investigated by evaluating of pyruvate dehydrogenase (PDH) and Lactate Dehydrogenase A (LDHA) protein expressions. Results indicated that, ZnO-NP application did not have a cytotoxic effect on HUVEC cells, it had cytotoxicity on both breast cancer cell lines. However, MCF7 cells were more sensitive to ZnO-NP treatment. Administration of 20 μg/mL ZnO-NP reduced the survival of MCF7 cells by 62% and increased the apoptotic index by approximately 6 times. Additionally, ZnO-NP treatment inhibited the doubling times of cells and suppressed the colony-forming abilities of both breast cancer cell lines. Also, it was seen that ZnO-NP treatment increased PDH expression in MCF7 cells, where the apoptotic index was more induced. As a result, we have shown for the first time that ZnO-NPs affect the energy metabolism of cells by increasing PDH expression in MCF7 cells, thus increasing the apoptotic index. Our study, which observed the anticancer effects of ZnO-NPs on breast cancer cells, will also shed light on future experimental studies. |
| Anahtar Kelimeler |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | Kafkas Universitesi Veteriner Fakultesi Dergisi |
| Dergi ISSN | 1300-6045 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q3 |
| Makale Dili | Türkçe |
| Basım Tarihi | 01-2024 |
| Cilt No | 30 |
| Sayı | 4 |
| Sayfalar | 489 / 496 |
| Doi Numarası | 10.9775/kvfd.2024.31688 |
| Makale Linki | http://dx.doi.org/10.9775/kvfd.2024.31688 |
| Atıf Sayıları |
