| Yazarlar |
Elif Karadeniz Pütür
|
Prof. Dr. Fatih BÜYÜK
Kafkas Üniversitesi, Türkiye |
|
Yaren Ersoy
Kafkas Üniversitesi, Türkiye |
| Özet |
| Among the heterogeneous Pasteurellaceae family, Pasteurella multocida and Mannheimia haemolytica are the pathogenic species that cause enzootic pneumonia in cattle and sheep. This study was designed to characterize Pasteurella and Mannheimia species obtained from pneumonia cases in cattle and sheep. The lungs of 100 cattle and 100 sheep diagnosed with infectious pneumonia as a result of histopathological examination were analyzed for Pasteurellaceae members by phenotypic methods, the VITEK 2 Compact system (VITEK), and polymerase chain reaction (PCR). Antibiotic susceptibilities and phylogenetic analysis of the isolates were done by VITEK and 16S rRNA-PCR, respectively. Seven Pasteurella multocida and 9 Mannheimia haemolytica were identified. All strains were resistant to ampicillin and trimethoprim/sulfamethoxazole. High susceptibility to ceftiofur (100%, 7/7 and 88.89%, 8/9) and doxycycline (85.71%, 6/7 and 100%, 9/9) was detected among the P. multocida and M. haemolytica isolates, respectively. The minimum inhibitory concentration (MIC) for gentamicin was within the epidemiologic cutoff limits. The diversity of resistance genes (TEM, OXA-58, AadB, AadA25, Tet H, and Sul 2) was high and their frequency ranged from 22.22% to 100% (16/16) among the strains. Multidrug resistance (MDR) was detected as 100% (7/7) for P. multocida and 33.33% (3/9) for M. haemolytica strains. Based on the MDR, six resistotypes (A to F) were determined, with more among the P. multocida strains. An approach called ‘antibiotic resistance marker-based genotyping’ was introduced based on the presence of antibiotic resistance genes, and 8 genotypes (I to VIII) were determined, with more profiles (I to V) among the P. multocida strains. The 16S rRNA gene sequencing positioned the strains in two main phylogenetic clusters, and P. multocida strains were found to be more genetically close. A better understanding of bacterial behaviors related to antimicrobial susceptibility may aid communication with producers and veterinarians regarding appropriate antimicrobial stewardship. Phylogenetic clustering of strains can increase country-specific sequence data and create new taxa from a universal perspective. |
| Anahtar Kelimeler |
| 16S rRNA sequencing | cattle | Pasteurellaceae | PCR | sheep | VITEK 2 Compact system |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | Turkish Journal of Veterinary and Animal Sciences |
| Dergi ISSN | 1300-0128 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q4 |
| Makale Dili | İngilizce |
| Basım Tarihi | 12-2024 |
| Cilt No | 48 |
| Sayı | 6 |
| Sayfalar | 227 / 242 |
| Doi Numarası | 10.55730/1300-0128.4360 |
| Makale Linki | https://journals.tubitak.gov.tr/cgi/viewcontent.cgi?article=4360&context=veterinary |
| Atıf Sayıları |
