Horseradish peroxidase immobilized in calcium alginate-gelatin hybrid beads with high stability against metallic ions and organic solvents
Yazarlar (4)
Doç. Dr. Kezban YILDIZ DALGINLI Kafkas Üniversitesi, Türkiye
Prof. Dr. Onur ATAKİŞİ Kafkas Üniversitesi, Türkiye
Öğr. Gör. Melek ÖZTÜRKLER DÜNDAR Kafkas Üniversitesi, Türkiye
Doç. Dr. Canan GÜLMEZ SAMSA Kafkas Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | CHEMICAL ENGINEERING COMMUNICATIONS (Q3) | ||
| Dergi ISSN | 0098-6445 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | Türkçe | Basım Tarihi | 08-2024 |
| Cilt / Sayı / Sayfa | 211 / 8 / 1222–1235 | DOI | 10.1080/00986445.2024.2341264 |
| Makale Linki | https://doi.org/10.1080/00986445.2024.2341264 | ||
| UAK Araştırma Alanları |
Biyokimya
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| Özet |
| Horseradish peroxidase (HRP) was immobilized in hybrid calcium alginate-gelatin hydrogel beads by entrapment. Then, organic solubility, metal ion compatibility, pH/temperature studies of the synthesized materials have been carried out. Hybrid beads (Ca-Alg@gel-HRP) were synthesized under optimum synthesis conditions with high yield and specific activity. The synthesized bead size was determined to be approximately 0.29 mm in diameter, hydrogel structure and transparent appearance. Accordingly, the optimum pH of free HRP and Ca-Alg@gel HRP was found to be pH 6 and 5 and the optimum temperature was found to be 40 °C and 60 °C respectively. For all identified metal ions except Fe2+, the Ca-Alg@gel HRP had a higher activity than the free HRP. In the presence of 1 mM Na+, Ca2+, Mg2+, Ba2+, Cu2+, Ni2+, Mn2+, Hg2+ ions, the activity increased by more than 20%. The relative activity was … |
| Anahtar Kelimeler |
| Enzyme | immobilization | hybrid beads | stability | organic synthesis | entrapment method |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 3 |
| Google Scholar | 3 |