Silver nanoparticles stimulate 5‐Fluorouracil‐induced colorectal cancer cells to kill through the upregulation TRPV1‐mediated calcium signaling pathways
Yazarlar (1)
Dr. Öğr. Üyesi Müge MAVİOĞLU KAYA Kafkas Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | CELL BIOLOGY INTERNATIONAL (Q3) | ||
| Dergi ISSN | 1065-6995 Dergi Bilgileri (2024) | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 05-2024 |
| Cilt / Sayı / Sayfa | 48 / 5 / 712–725 | DOI | 10.1002/cbin.12141 |
| Makale Linki | https://doi.org/10.1002/cbin.12141 | ||
| UAK Araştırma Alanları |
Biyoloji
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| Özet |
| The involvement of the TRP vanilloid 1 (TRPV1) cation channel on the 5-Fluorouracil (5-FU)-caused Ca2+ signals through the activation of the apoptotic signaling pathway and stimulating the mitochondrial Ca2+ and Zn2+ accumulation-induced reactive oxygen species (ROS) productions in several cancer cells, except the colorectal cancer (HT-29) cell line, was recently reported. I aimed to investigate the action of silver nanoparticles (SiNPs) and 5-FU incubations through the activation of TRPV1 on ROS, apoptosis, and cell death in the HT-29 cell line. The cells were divided into four groups: control, SiNP (100 µM for 48 h), 5-FU (25 μM for 24 h), and 5-FU + SiNP. SiNP treatment through TRPV1 activation (via capsaicin) stimulated the oxidant and apoptotic actions of 5-FU in the cells, whereas they were diminished in the cells by the TRPV1 antagonist (capsazepine) treatment. The apoptotic and cell death actions of 5-FU were determined by increasing the propidium iodide/Hoechst rate, caspase-3, -8, and -9 activations, mitochondrial membrane depolarization, lipid peroxidation, and ROS, but decreasing the glutathione and glutathione peroxidase. The increase of cytosolic free Ca2+ and Zn2+ into mitochondria via the stimulation of TRPV1 current density increased oxidant and apoptotic properties of 5-FU in the cells. For the therapy of HT-29 tumor cells, I found that the combination of SiNPs and 5-FU was synergistic via TRPV1 activation. |
| Anahtar Kelimeler |
| 5‐Fluorouracil | TRPV1 channel | apoptosis | colorectal cancer | mitochondria | silver nanoparticles |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Yöksis | 5 |
| Web of Science | 8 |
| PubMed | 2 |