The Protective Effects of a Single Dose Myricetin Application on CLP-Induced Rat Sepsis Model by Analyzing Some Immune Mechanisms
Yazarlar (7)
Doç. Dr. İsmail CAN
Kafkas Üniversitesi, Türkiye
Ali Güraslan
Dr. Öğr. Üyesi Ömer Faruk BAŞER
Kafkas Üniversitesi, Türkiye
Gülfem Nur Yıldız
Dr. Öğr. Üyesi İhsan TOPALOĞLU
Kafkas Üniversitesi, Türkiye
Prof. Dr. Selina AKSAK KARAMEŞE
Kafkas Üniversitesi, Türkiye
Doç. Dr. Murat KARAMEŞE
Kafkas Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY (Q2) | ||
| Dergi ISSN | 0892-3973 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | Türkçe | Basım Tarihi | 05-2025 |
| Cilt / Sayı / Sayfa | 47 / 3 / 305–316 | DOI | 10.1080/08923973.2025.2469227 |
| Makale Linki | https://doi.org/10.1080/08923973.2025.2469227 | ||
| Özet |
| In this study, our aim was to investigate the protective effects of myricetin (single dose-100 mg/kg) on CLP-induced rat sepsis model by analyzing some immune mechanisms including inflammation and oxidative stress by different techniques such as Immunohistochemistry, ELISA, tissue biochemistry and Western Blotting. Twenty-eight Wistar albino rats were divided into 4 groups. The pro-inflammatory and anti-inflammatory cytokine levels were measured by ELISA technique. CD68 and Nuclear-Factor-Kappa-B (NF-κB) positivity rates were detected by IHC. Some of oxidative stress parameters were measured by tissue biochemistry, while Toll-like receptor-4 (TLR4) expression others were detected by Western blot technique. Sepsis caused a significant increase in all pro-inflammatory cytokine and oxidant levels. Also, it led to an increase in the positivity of CD68 and NF-κB markers as well as the expression levels of TNF-alpha, IL-1-beta, TLR4, Keap-1. However, single dose myricetin application normalized pro-inflammatory cytokine levels, increased anti-oxidant and anti-inflammatory cytokine levels, decreased positivity of CD68 and NF-κB and increased NRF2 and HO-1 expressions. As a conclusion, the beneficial effect of myricetin on lung injury also involved inhibition of TLR4/NF-κB pathway, suppression of proinflammatory cytokines and induction of anti-inflammatory cytokine production, regulation of oxidant and anti-oxidant system parameters, and activating the NRF2/Keap1/HO-1 pathway. |
| Anahtar Kelimeler |
| Sepsis | CLP | myricetin | flavonoids | inflammation | oxidative stress |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| WoS | 4 |