| Yazarlar |
Seda Tezcan Ülger
Mersin Üniversitesi, Türkiye |
Dr. Öğr. Üyesi Didem ÖZGÜR
Mersin Üniversitesi, Türkiye |
|
Mahmut Ülger
Mersin Üniversitesi, Türkiye |
|
Gönül Aslan
Mersin Üniversitesi, Türkiye |
|
İclal Gürses
Mersin Üniversitesi, Türkiye |
|
Mehmet Sami Serin
Mersin Üniversitesi, Türkiye |
|
Burcu Gürer Giray
Türkiye |
|
Gürol Emekdaş
Biruni Üniversitesi, Türkiye |
| Özet |
| Infection with certain human papillomavirus (HPV) genotypes is the most important risk factor related with cervical cancer. The objective of the present study was to investigate the prevalence of HPV infection, the distribution of HPV genotypes and HPV E6/E7 oncogene mRNA expression in Turkish women with different cervical cytological findings in Mersin province, Southern Turkey. A total of 476 cytological samples belonging to women with normal and abnormal cervical Pap smears were enrolled in the study. For the detection and genotyping assay, a PCR/direct cycle sequencing approach was used. E6/E7 mRNA expression of HPV-16, 18, 31, 33, and 45 was determined by type-specific real-time NASBA assay (NucliSENS EasyQ(®)HPV v1.1). Of the 476 samples, 106 (22.3%) were found to be positive for HPV DNA by PCR. The presence of HPV was significantly more common (p<0.001) in HSIL (6/8, 75%) when compared with LSIL (6/14, 42.9%), ASC-US (22/74, 29.7%) and normal cytology (72/380, 18.9%). The most prevalent genotypes were, in descending order of frequency, HPV genotype 66 (22.6%), 16 (20.8%), 6 (14.2%), 31 (11.3%), 53 (5.7%), and 83 (4.7%). HPV E6/E7 oncogene mRNA positivity (12/476, 2.5%) was lower than DNA positivity (38/476, 7.9%). Our data present a wide distribution of HPV genotypes in the analyzed population. HPV genotypes 66, 16, 6, 31, 53 and 83 were the predominant types and most of them were potential carcinogenic types. Because of the differences between HPV E6/E7 mRNA and DNA positivity, further studies are required to test the role of mRNA testing in the triage of women with abnormal cervical cytology or follow up of HPV DNA positive and cytology negative. These epidemiological data will be important to determine the future impact of vaccination on HPV infected women in our region. |
| Anahtar Kelimeler |
| Human papillomavirus | consensus PCR | cycle sequencing | E6/E7 mRNA | genotype | Turkish women |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | ASIAN PACIFIC JOURNAL OF CANCER PREVENTION |
| Dergi ISSN | 1513-7368 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q3 |
| Makale Dili | İngilizce |
| Basım Tarihi | 1-2014 |
| Cilt No | 15 |
| Sayı | 9 |
| Sayfalar | 3997 / 4003 |
| Doi Numarası | 10.7314/APJCP.2014.15.9.3997 |
| Makale Linki | http://koreascience.or.kr/journal/view.jsp?kj=POCPA9py=2014vnc=v15n9sp=3997 |
| Atıf Sayıları | |
| WoS | 16 |
