Molecular Characterization of Insect-Borne (Mosquito-Sandfly) Parasitic Pathogens of Dogs in Selected Regions of Northeast Anatolia, Türkiye
Yazarlar (7)
Doç. Dr. Nilgün AYDIN Kafkas Üniversitesi, Türkiye
Dr. Öğr. Üyesi Neslihan ÖLMEZ Kafkas Üniversitesi, Türkiye
Prof. Dr. Barış SARI Kafkas Üniversitesi, Türkiye
Prof. Dr. Zati VATANSEVER Kafkas Üniversitesi, Türkiye
Prof. Dr. Gencay Taşkın TAŞÇI Kafkas Üniversitesi, Türkiye
Dr. Öğr. Üyesi Şemistan Kızıltepe Iğdır Üniversitesi, Türkiye
Arş. Gör. Mesut YİĞİT Kafkas Üniversitesi, Türkiye
Makale Türü Açık Erişim Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı Pakistan Veterinary Journal (Q1)
Dergi ISSN 0253-8318 Dergi Bilgileri (2025)
Dergi Tarandığı Indeksler SCI-Exp, SCOPUS, Curation, Essential Science Indicators, Pdf2xml, Pdf2xml, Reference Master, Sophia, Web Of Science Expanded, Zoological Record
Makale Dili İngilizce Basım Tarihi 01-2025
Cilt / Sayı / Sayfa 45 / 1 / 304–311 DOI 10.29261/pakvetj/2025.112
Makale Linki https://doi.org/10.29261/pakvetj/2025.112
UAK Araştırma Alanları
Veteriner Parazitolojisi
Özet
Leishmania spp. Examination of dog blood samples from randomly selected focal areas was undertaken using the conventional PCR method. The molecular prevalence of these parasitic pathogens and the correlation between sex, age, breed and habitat of dogs were ascertained by employing the Pearson Chi-Square Test. The overall prevalence of D. immitis was 5%(25/500), while the PCR detected DNAs in dogs in Kars, Ardahan, and Iğdır at 6.08%(CI: 3.53-9.73), 4.03%(CI: 1.55-8.61), and 4.10%(CI: 1.73-8.27), respectively. Portions of the target genes amplified from positive samples were sequenced to confirm the PCR results. The outcomes were aligned with additional sequences in the NCBI database using BLAST search. Using the Bayesian Inference method with 2 million generations (MCMC criteria: 50,000 burn-inlength), phylogenetic trees were reconstructed using MrBayes v3. 2.6. Furthermore, this study did not detect Leishmania spp., D. repens and A. reconditum DNAs. Knowing the existence and prevalence of insect-borne pathogens in dogs is most important and necessary for establishing a treatment protocol and determining protection and control measures.
Anahtar Kelimeler
Dirofilaria | Dog | Insect-borne pathogens | Molecular Epidemiology