| Yazarlar (7) |
Ismail Can
|
|
Ali Guraslan
|
Arş. Gör. Ömer Faruk BAŞER
Kafkas Üniversitesi, Türkiye |
|
Gulfem Nur Yildiz
|
|
Ihsan Toplaoglu
|
|
Selina Aksak Karamese
|
Doç. Dr. Murat KARAMEŞE
Kafkas Üniversitesi, Türkiye |
| Özet |
| In this study, our aim was to investigate the protective effects of myricetin (single dose-100 mg/kg) on CLP-induced rat sepsis model by analyzing some immune mechanisms including inflammation and oxidative stress by different techniques such as Immunohistochemistry, ELISA, tissue biochemistry and Western Blotting. Twenty-eight Wistar albino rats were divided into 4 groups. The pro-inflammatory and anti-inflammatory cytokine levels were measured by ELISA technique. CD68 and Nuclear-Factor-Kappa-B (NF-κB) positivity rates were detected by IHC. Some of oxidative stress parameters were measured by tissue biochemistry, while Toll-like receptor-4 (TLR4) expression others were detected by Western blot technique. Sepsis caused a significant increase in all pro-inflammatory cytokine and oxidant levels. Also, it led to an increase in the positivity of CD68 and NF-κB markers as well as the expression levels of TNF-alpha, IL-1-beta, TLR4, Keap-1. However, single dose myricetin application normalized pro-inflammatory cytokine levels, increased anti-oxidant and anti-inflammatory cytokine levels, decreased positivity of CD68 and NF-κB and increased NRF2 and HO-1 expressions. As a conclusion, the beneficial effect of myricetin on lung injury also involved inhibition of TLR4/NF-κB pathway, suppression of proinflammatory cytokines and induction of anti-inflammatory cytokine production, regulation of oxidant and anti-oxidant system parameters, and activating the NRF2/Keap1/HO-1 pathway. |
| Anahtar Kelimeler |
| Sepsis | CLP | myricetin | flavonoids | inflammation | oxidative stress |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | ESCI dergilerinde yayımlanan tam makale |
| Dergi Adı | IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY |
| Dergi ISSN | 0892-3973 Wos Dergi Scopus Dergi |
| Makale Dili | İngilizce |
| Basım Tarihi | 03-2025 |
| Doi Numarası | 10.1080/08923973.2025.2469227 |
| Atıf Sayıları |
