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A Mixed Hemadsorption Assay for Detection of Cell Surface Binding Anti tumor Antibodies in Human Sera       
Yazarlar (3)
Prof. Dr. Neşe SANSA Prof. Dr. Neşe SANSA
The Wistar Institute, Amerika Birleşik Devletleri
Claudia Andl
The Wistar Institute, Amerika Birleşik Devletleri
Dawei Zhou
The Wistar Institute, Amerika Birleşik Devletleri
Devamını Göster
Özet
A practical mixed hemadsorption assay (MHA) was developed for the detection of cell surface binding anti-tumor antigen antibodies (anti-TA Ab) in the human sera. The assay was compared to enzyme-linked immunosorbent assay (ELISA) and dot ELISA in the detection of the antibodies developed against GA733-2 antigen. SW1116 cell line and recombinant GA733-2E protein were used as targets in the MHA and solid phase immunoassays (SPIA), respectively. Sera were obtained from healthy donors and vaccinated-colon carcinoma patients. The sensitivity level of the MHA was very high for detection of anti-TA Ab in sera. © 2002 Elsevier Science B.V. All rights reserved.
Anahtar Kelimeler
GA733 | Humoral response in cancer | Mixed hemadsorption assay
Makale Türü Özgün Makale
Makale Alt Türü Diğer hakemli uluslarası dergilerde yayınlanan tam makale
Dergi Adı Journal of Immunological Methods
Dergi ISSN 0022-1759 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler EBSCO
Makale Dili İngilizce
Basım Tarihi 01-2002
Cilt No 261
Sayı 1
Sayfalar 119 / 127
Doi Numarası 10.1016/S0022-1759(01)00560-9