| Yazarlar (4) |
Prof. Dr. Neşe SANSA
Haliç Üniversitesi |
Osman Ziya Sayhan
Marmara Üniversitesi, Türkiye |
|
Ali Karaçavuş
|
|
Kurtuluş Töreci
|
| Özet |
| S. typhimurium was acetone dried, negative stained, and its outer membrane was evaluated by transmission electron microscopy. White Albino rabbits were immunized against acetone derived S. typhimurium, and the immune sera were collected. Outer membrane antigens were extracted from acetone dried and veronal buffer treated S. typhimurium, S. typhi, S. paratyphi-B, E. coli, and S. flexineri. S. typhimurium extract was further fractionated. Immunogenicity and cross reactivity of each antigenic fraction were evaluated by ELISA and immunodiffusion using the immune sera and commercially available or in-house product sera. Molecular sizes of proteins in fractions were evaluated using SDS-PAGE. A S. typhimurium specific 70 kDa antigen was detected. However, highest antigenicity was detected in 36-43 kDa common protein fraction. These antigenic fractions should have utility in medical microbial immunology. |
| Anahtar Kelimeler |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | Diğer hakemli ulusal dergilerde yayınlanan tam makale |
| Dergi Adı | J Cell Mol Biol |
| Dergi Tarandığı Indeksler | EBSCO |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2003 |
| Cilt No | 2 |
| Sayfalar | 91 / 96 |
