| Yazarlar (2) |
Dr. Öğr. Üyesi Nurullah ÇİFTÇİ
Kafkas Üniversitesi, Türkiye |
Doç. Dr. Zihni Onur UYGUN
Kafkas Üniversitesi, Türkiye |
| Özet |
| This study aimed to develop a rapid and highly sensitive electrochemical biosensor for the sequence-specific detection of Escherichia coli 16S rRNA using a peptide nucleic acid (PNA) probe immobilized on a graphene - NH/gold-nanoparticle (GNH - AuNP) composite. An SH-terminated PNA probe was covalently attached to a GNH - AuNP nanocomposite layer deposited on gold screen-printed electrodes via 3-mercaptopropionic acid (MPA). The electrode assembly and hybridization processes were characterized by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and X-ray photoelectron spectroscopy (XPS). Quantification was achieved using differential pulse voltammetry (DPV) with hexaammineruthenium(III) (RuHex) as an electrostatic redox indicator. Hybridization of the target 16S rRNA sequence produced a distinct "signal-on" DPV response proportional to target concentration. The biosensor exhibited excellent linearity from 1 to 1000 pM (R = 0.9984), with a limit of detection (LOD) of 0.21 pM and a limit of quantification (LOQ) of 0.70 pM. The neutral-backbone PNA probe improved hybridization kinetics and selectivity. The PNA/GNH - AuNP interface enabled fast, stable, and highly sensitive E. coli detection. Its disposable electrode format and portable electrochemical readout make it suitable for on-site diagnostic and food-safety monitoring applications. |
| Anahtar Kelimeler |
| Biosensor | peptide nucleic acid | E. coli | differential pulse voltammetry | graphene |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale |
| Dergi Adı | Nanomedicine |
| Dergi ISSN | 1743-5889 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q2 |
| Makale Dili | Türkçe |
| Basım Tarihi | 10-2025 |
| Sayfalar | 1 / 11 |
| Doi Numarası | 10.1080/17435889.2025.2577218 |
| Makale Linki | https://doi.org/10.1080/17435889.2025.2577218 |
| Atıf Sayıları |
