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Low dosages of vitamin A may cause a decrease in the total neuron number of fetal hippocampal rat cells     
Yazarlar
Hakan Ay
Türkiye
Dr. Öğr. Üyesi Duygu ASLAN Dr. Öğr. Üyesi Duygu ASLAN
Türkiye
Erdem Söztutar
Türkiye
Ferruh Yücel
Eskişehir Osmangazi Üniversitesi, Türkiye
Özet
We investigated the effect of low, medium and high doses of oral vitamin A, on the number of fetal hippocampal neurons. High doses of vitamin A during pregnancy may cause embryonic malformations. There are reports about dosages that don't cause macroscopic malformations, but may cause mental and behavioral disorders. Still, quantitative morphological studies explaining this topic are lacking. We administered oral vitamin A to pregnant rats on the 10th-12th days of pregnancy at doses of 10000, 20000, 30000, 40000, 50000, 100000 and 200000 IU/kg. We collected the fetuses on the 19th day and removed their brains. After staining with cresyl violet and immunolabeling with Tunel and Ki67 antibody, we examined the hippocampi with stereological methods. Vitamin A decreased hippocampal neuron numbers beginning from 20000 IU/kg. While the number of Ki67 positive cells increased with the dosage, the increase of apoptotic cells begun at the dose of 50000 IU/kg. Our study demonstrates that vitamin A, beginning from the dosage of 20000 IU/kg, is decreasing the total hippocampal neuron numbers during the critical period of embryonic brain development and that apoptosis may not be the only factor in this outcome (Tab. 1, Fig. 3, Ref. 27).
Anahtar Kelimeler
vitamin A | hippocampus | neuron number | stereology
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı Bratislava Medical Journal
Dergi ISSN 1336-0345
Dergi Tarandığı Indeksler SCI-Expanded
Dergi Grubu Q3
Makale Dili İngilizce
Basım Tarihi 01-2020
Cilt No 121
Sayı 8
Sayfalar 580 / 583
Doi Numarası 10.4149/BLL_2020_096
Makale Linki http://dx.doi.org/10.4149/bll_2020_096