The beneficial effects of dose-dependent myricetin application on renal ischemia and reperfusion injury in a rat model
Yazarlar (6)
Dr. Öğr. Üyesi Mert SEZER Kafkas Üniversitesi, Türkiye
Dr. Öğr. Üyesi Yusuf Umut BATI Kafkas Üniversitesi, Türkiye
Nazlı Yerli
Dr. Öğr. Üyesi Soner KINA Kafkas Üniversitesi, Türkiye
Dr. Öğr. Üyesi Lale BAŞER Kafkas Üniversitesi, Türkiye
Prof. Dr. Selina AKSAK KARAMEŞE Kafkas Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Molecular Biology Reports (Q3) | ||
| Dergi ISSN | 0301-4851 Dergi Bilgileri (2025) | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 09-2025 |
| Cilt / Sayı / Sayfa | 52 / 1 / 905– | DOI | 10.1007/s11033-025-11024-x |
| Makale Linki | https://doi.org/10.1007/s11033-025-11024-x | ||
| UAK Araştırma Alanları |
Anesteziyoloji ve Reanimasyon
|
||
| Özet |
| This study was aimed to evaluate the renoprotective effects of Myricetin at two dosages (50 and 100 mg/kg) within an experimental I/R injury model. The analysis focused on key immunological and oxidative stress pathways, including the regulation of pro- and anti-inflammatory cytokines, NF-κB and TLR4 signaling, as well as biomarkers of oxidative stress such as nitrate (NO), malondialdehyde (MDA), glutathione (GSH), nuclear factor (erythroid-derived 2)-like 2 (NRF2) and Heme oxygenase-1 (HO-1). Thirty female Wistar albino rats were randomly assigned to six groups. Myricetin was administered via intragastric gavage for seven days before ischemia/reperfusion. Renal ischemia was induced by clamping the left renal pedicle for 45 min, followed by a 3-hour reperfusion period. Kidney tissues were collected for histopathological, immunohistochemical, and biochemical analyses. Serum cytokine levels were measured by ELISA, while protein expressions were evaluated by Western blotting. I/R injury significantly increased pro-inflammatory cytokines and oxidative stress markers, alongside heightened NF-κB immunopositivity and TNF-α, IL-1β expression. Myricetin administration reduced pro-inflammatory cytokines, increased anti-inflammatory cytokines, and enhanced antioxidant responses. Immunohistochemical and Western blot analyses showed decreased NF-κB positivity and increased NRF2 expression. The renoprotective effects of Myricetin appear to be mediated by inhibiting the NF-κB pathway, reducing inflammation, and enhancing antioxidant responses. Furthermore, Myricetin activates the NRF2/HO-1 pathway, suggesting its potential as a therapeutic agent for ischemia/reperfusion-induced kidney injury. |
| Anahtar Kelimeler |
| Renal ischemia/Reperfusion | Myricetin | Apoptosis | Oxidative stress | Cytokines |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Yöksis | 1 |
| Web of Science | 3 |
| PubMed | 1 |