Immunohistochemical Investigation of Oxidative Stress-induced DNA Damage and Lipid Peroxidation in Bovine Papillomas and Fibropapillomas
Yazarlar (1)
Doç. Dr. Emin KARAKURT Kafkas Üniversitesi, Türkiye
| Makale Türü |
Özgün Makale
(Diğer hakemli uluslarası dergilerde yayınlanan tam makale)
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| Dergi Adı | Van Veterinary Journal | ||
| Dergi ISSN | 2149-3359 | ||
| Dergi Tarandığı Indeksler | Sobiad, CAB Abstracts, Ebscohost, Index Copernicus, Türkiye Atıf Dizini, Google Scholar, Asos Index | ||
| Makale Dili | İngilizce | Basım Tarihi | 03-2021 |
| Cilt / Sayı / Sayfa | 32 / 1 / 22–27 | DOI | 10.36483/vanvetj.843747 |
| Makale Linki | https://dergipark.org.tr/tr/pub/vanvetj/issue/60883/843747 | ||
| UAK Araştırma Alanları |
Veterinerlik Patolojisi
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| Özet |
| In this study, it was aimed to evaluate the expressions of 8-OHdG and MDA immunohistochemically in order to determine the oxidative stress-induced DNA damage and lipid peroxidation in 16 papillomas and 14 fibropapillomas from 30 cattle brought to our department between 2013-2020. Biopsy samples taken after surgery were fixed in buffered 10% formaldehyde solution. Sections of 5 μm thickness were taken from the paraffin blocks prepared after routine tissue follow-up procedures and Hematoxylin & Eosin staining was applied to the sections in order to detect histopathological changes. Avidin Biotin Peroxidase method was used for immunohistochemical staining. In papilloma cases, severe hyperkeratosis, retepects extending from the epidermis to the dermis, spongiosis and balloon-like degeneration in squamous epithelial cells, basophilic inclusion bodies in granular cells, ulcerations in the epidermis layer, hemorrhagic areas and an increase in kerato hyaline granules were observed. In addition to these findings, dense connective tissue increases were detected in fibropapilloma cases. In immunohistochemical evaluations, positive reactions for BPV were observed in the nuclei of cells in stratum granulosum. 8-OHdG positive reactions were detected in the cytoplasm and nucleus of the epidermal cells in papilloma cases, whereas in cases of fibropapilloma, reactions in these cells were observed in the cytoplasm of fibrocytes and fibroblasts in the dermis. Membranous MDA positive reactions were observed in the epidermal cells in papilloma cases, whereas MDA expressions were detected in the cytoplasm of fibrocytes and fibroblasts in … |
| Anahtar Kelimeler |
| lipid peroxidation | oxidative stress | DNA damage | papilloma |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Google Scholar | 3 |