Immunohistochemical and Molecular Detection of Mannheimia spp.and Pasteurella spp. in Sheep with Pneumonia in Kars Province - Turkey
Yazarlar (8)
Prof. Dr. Serpil DAĞ Kafkas Üniversitesi, Türkiye
Abdurrahman Gürbüz
Kafkas Üniversitesi, Türkiye
Kafkas Üniversitesi, Türkiye
Prof. Dr. Hasan Özen Kafkas Üniversitesi, Türkiye
Prof. Dr. Fatih BÜYÜK Kafkas Üniversitesi, Türkiye
Prof. Dr. Özgür ÇELEBİ Kafkas Üniversitesi, Türkiye
Prof. Dr. Musa Karaman Balıkesir Üniversitesi, Türkiye
Prof. Dr. Mehmet Çitil Erciyes Üniversitesi, Türkiye
Doç. Dr. Emin KARAKURT Kafkas Üniversitesi, Türkiye
| Makale Türü |
Özgün Makale
(SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
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| Dergi Adı | Kafkas Universitesi Veteriner Fakultesi Dergisi (Q4) | ||
| Dergi ISSN | 1300-6045 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | Türkçe | Basım Tarihi | 03-2018 |
| Cilt / Sayı / Sayfa | 24 / 2 / 281–288 | DOI | 10.9775/kvfd.2017.18840 |
| Makale Linki | http://vetdergikafkas.org/abstract.php?lang=en&id=2320 | ||
| UAK Araştırma Alanları |
Veteriner Patolojisi
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| Özet |
| In this investigation, presence of Mannheimia haemolytica and Pasteurella multocida in sheep brought to Kafkas University Faculty of Veterinary Medicine between 2011 and 2013 with a suspicion of pneumonia was studied by immunohistochemical and bacteriological methods. Study materials were 100 sheep lungs. After routine histopathological investigations lungs were immunohistochemically stained with antibodies prepared against both of the agents. In bacteriological investigations, tissue samples were inoculated into 7% sheep blood agar and Mc Conkey agar and incubated for 24-48 h in aerobic conditions, and the suspected colonies were evaluated for Mannheimia and Pasteurella species. In histological investigations, pneumonias were classified as 28% fibrinous bronchopneumonia, 21% prulent bronchopneumonia, 20% acute-catharal bronchopneumonia, 18% interstitial pneumonia, 11% verminous pneumonia and 2% adenomatous pneumonia. In bacteriological investigations Mannheimia haemolytica and Pasteurella multocida was detected in 19 and 3 cases, respectively, these numbers were 17 and 2, respectively in immunohistochemical investigations. The results of PCR analysis for both Mannheimia haemolytica and Pasteurella multocida paralleled with the findings of microbiological culture. The results of the study showed that Mannheimia haemolytica is an important cause of pneumonia in sheep in Kars, and Pasteurella multocida, although with lesser importance can cause pneumonia in this species. |
| Anahtar Kelimeler |
| Immunohistochemistry | Mannheimia haemolytica | Pasteurella multocida | Pneumonia | Sheep |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 5 |
| Scopus | 8 |
| Google Scholar | 17 |