Assessment of MDA and 8-OHdG expressions in ovine pulmonary adenocarcinomas by immunohistochemical and immunofluorescence methods
Yazarlar (6)
Doç. Dr. Emin KARAKURT Kafkas Üniversitesi, Türkiye
Prof. Dr. Enver BEYTUT Kafkas Üniversitesi, Türkiye
Prof. Dr. Serpil DAĞ Kafkas Üniversitesi, Türkiye
Dr. Öğr. Üyesi Hilmi NUHOĞLU Kafkas Üniversitesi, Türkiye
Dr. Öğr. Üyesi Ayfer YILDIZ UYSAL Kafkas Üniversitesi, Türkiye
Emre Kurtbaş Kafkas Üniversitesi, Veteriner Fakültesi
| Makale Türü |
Özgün Makale
(SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
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| Dergi Adı | Acta Veterinaria Brno (Q4) | ||
| Dergi ISSN | 1801-7576 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 07-2022 |
| Kabul Tarihi | 12-04-2026 | Yayınlanma Tarihi | – |
| Cilt / Sayı / Sayfa | 91 / 3 / 235–241 | DOI | 10.2754/avb202291030235 |
| Makale Linki | https://actavet.vfu.cz/91/3/0235/ | ||
| Özet |
| This study aimed to reveal the presence of reactive oxygen species (ROS)-induced lipid peroxidation and DNA damage in ovine pulmonary adenocarcinomas (OPA) by evaluating malondialdehyde (MDA) and 8-hydroxy-2-deoxyguanosine (8-OHdG) expressions by immunohistochemical and immunofluorescence methods. Lung tissue samples were collected from 26 sheep brought to the Pathology Department for routine diagnosis. Lung tissues were fixed in 10% neutral buffered formalin, following routine procedures tissues were stained with hematoxylin and eosin. Avidin-Biotin Peroxidase method was used as immunohistochemical staining. Indirect immunofluorescence method was applied to the sections. Tumoral cells showed acinar, papillary or mixed type patterns. Only 2 of 20 cases metastasized to regional lymph nodes. All OPAs were immune positive for Jaagsiekte Sheep Retrovirus Capsid Protein (JSRV CA), MDA and 8-OHdG. The control group was negative for JSRV CA, MDA and 8-OHdG expressions. Malondialdehyde and 8-OHdG immune positive cells were statistically increased in the OPA group compared to the control group. In conclusion, our results demonstrate that higher MDA and 8-OHdG expressions in sheep with OPA suggest that OPA may be closely related to lipid peroxidation and oxidative DNA damage. |
| Anahtar Kelimeler |
| DNA damage | jaagsiekte | lipid peroxidation | oxidative stress | sheep |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Google Scholar | 8 |
| Web of Science | 6 |
| Scopus | 7 |