Immunohistochemical and Microbiological Detection of Brucella abortus in Aborted Bovine Fetuses
Yazarlar (5)
M. Sözmen
Kafkas Üniversitesi, Veteriner Fakültesi, Türkiye
Kafkas Üniversitesi, Veteriner Fakültesi, Türkiye
S. D. Erginsoy
Kafkas Üniversitesi, Veteriner Fakültesi, Türkiye
Kafkas Üniversitesi, Veteriner Fakültesi, Türkiye
O. Genç
Kafkas Üniversitesi, Veteriner Fakültesi, Türkiye
Kafkas Üniversitesi, Veteriner Fakültesi, Türkiye
Prof. Dr. Enver BEYTUT Kafkas Üniversitesi, Türkiye
K. Özcan
Kafkas Üniversitesi, Veteriner Fakültesi, Türkiye
Kafkas Üniversitesi, Veteriner Fakültesi, Türkiye
| Makale Türü |
Özgün Makale
(SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
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| Dergi Adı | Acta Veterinaria Brno (Q3) | ||
| Dergi ISSN | 0001-7213 Dergi Bilgileri (2004) | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 12-2004 |
| Cilt / Sayı / Sayfa | 73 / 4 / 465–472 | DOI | 10.2754/avb200473040465 |
| Makale Linki | http://actavet.vfu.cz/73/4/0465/ | ||
| UAK Araştırma Alanları |
Veterinerlik Patolojisi
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| Özet |
| Materials and MethodsHistopathology and immunohistochemistry The study was done on tissues from 25 aborted bovine fetuses. These fetuses were submitted to the Pathology Department, University of Kafkas, Kars, Turkey for necropsy between December 1998 and February 2002. None of the herds that aborted fetuses come from vaccinated against the Brucella abortus infection. Tissues examined included fetal lungs, liver, heart, kidneys, spleen, brain, bronchial lymph node, thymus, and abomasum. Specimens were fixed in 10% neutral buffered formalin, processed routinely for histopatologic analysis. All sections were stained with hematoxylin and eosin.For the immunohistochemical analysis, sections of 4 µm thickness were treated with freshly prepared 3% H2O2 in methanol for 15 min. to remove endogeneous peroxidase activity. After three rinses with phosphate-buffered saline (PBS), all tissue sections were incubated with 5% normal goat serum (Dako, Carpinteria, USA). An anti-B. abortus polyclonal antibody (Difco lab., Detroit, MI), prepared in rabbit and diluted 1: 100 in PBS with 2% normal goat serum was applied overnight at 4 C, was used. After three rinses with PBS, sections were incubated with biotinylated goat anti-rabbit immunoglobulin G (Dako, Carpinteria, USA) diluted 1: 200 in PBS for 60 min at room temperature. Sections then were incubated with streptavidin peroxidase complex (ABC; Dako, Carpinteria, USA). The peroxidase was localized with DAB chromogen. Sections were counterstained with Mayer’s hematoxylin, dehydrated and mounted. Control sections were incubated with normal rabbit serum instead of primary … |
| Anahtar Kelimeler |
| Abortion | Biotyping | Bovine fetus | Brucella abortus | Immunoperoxidase |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 15 |
| Scopus | 16 |
| Google Scholar | 32 |