Increased plasma cardiac troponin I concentration in lambs with myocarditis
Yazarlar (6)
Tolga Karapınar Fırat Üniversitesi, Türkiye
Yesari Eröksüz Fırat Üniversitesi, Türkiye
Prof. Dr. Enver BEYTUT Kafkas Üniversitesi, Türkiye
İbrahim Sözdutmaz Erciyes Üniversitesi, Türkiye
Hatice Eröksüz Fırat Üniversitesi, Türkiye
Murat Dabak Fırat Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Veterinary Clinical Pathology | ||
| Dergi ISSN | 0275-6382 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 09-2012 |
| Cilt / Sayı / Sayfa | 41 / 3 / 375–381 | DOI | 10.1111/j.1939-165X.2012.00448.x |
| Makale Linki | http://doi.wiley.com/10.1111/j.1939-165X.2012.00448.x | ||
| Özet |
| BackgroundCardiac troponin I (cTnI) is a blood biomarker of myocardial injury. A human cTnI assay may be useful for measuring cTnI concentrations in lambs with naturally occurring myocarditis.ObjectiveThe aims of this study were to evaluate the utility of a commercially available human chemiluminescent microparticle cTnI immunoassay for measuring plasma cTnI concentrations in lambs with naturally occurring myocarditis from infection with foot and mouth disease virus (FMDV), and to determine cTnI expression in cardiac muscle of affected lambs.MethodsTen lambs with myocarditis and 10 clinically healthy lambs (control group) were included. Clinical signs, gross and histologic necropsy findings, and immunoreactivity for cTnI in cardiac tissue were evaluated. Plasma cTnI concentration was determined using the commercial human immunoassay system.ResultsAll lambs with myocarditis died within 1 day of clinical signs. Infection with FMDV was confirmed by PCR analysis. Gross cardiac lesions were evident and histologic examination revealed myocarditis. Immunoreactivity for cTnI was absent in cardiac myocytes that were degenerative or necrotic, but was strong in cardiac myocytes from unaffected areas of the myocardium and in all cardiac myocytes of healthy lambs. The geometric mean plasma concentrations of cTnI for lambs in the myocarditis and control groups were 146.78 μg/L (95% confidence interval [CI], 61.90–348.06) and 0.013 μg/L (95% CI, 0.010–0.017), respectively (t‐value 19.27; P < .0001).ConclusionsA commercial human cTnI assay may be used to detect plasma cTnI concentrations in sheep, and cTnI may be used as a blood‐based biomarker of myocarditis in this species. |
| Anahtar Kelimeler |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları |