Molecular identification and hydrolase activity profiling of bacteria isolated from septic surface environments using 16S rRNA sequencing
 
Yazarlar (2)
Öğr. Gör. Esra Albayrak Kafkas Üniversitesi, Türkiye
Dr. Öğr. Üyesi Orhan ULUÇAY Kafkas Üniversitesi, Türkiye
Makale Türü Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı Molecular Biology Reports (Q3)
Dergi ISSN 0301-4851 Dergi Bilgileri (2026)
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce Basım Tarihi 12-2026
Cilt / Sayı / Sayfa 53 / 1 / – DOI 10.1007/s11033-026-11957-x
Makale Linki https://doi.org/10.1007/s11033-026-11957-x
UAK Araştırma Alanları
Bakteriyoloji Enzim ve Mikrobiyal Biyoteknoloji Mikrobiyoloji
Özet
BackgroundHospital septic surfaces represent critical reservoirs for persistent microbial contamination and may contribute to the spread of opportunistic pathogens in healthcare environments. Enzyme-producing bacteria colonizing such surfaces can enhance environmental persistence and resistance to routine sanitation procedures. This study aimed to investigate the diversity, enzymatic potential, and phylogenetic relationships of bacteria isolated from hospital septic surfaces using morphological, biochemical, and molecular approaches.MethodsEnvironmental samples were collected from selected hospital septic surfaces, and bacterial isolates were obtained using standard microbiological techniques. Morphological and biochemical characterization was performed using conventional assays, including catalase, protease, lipase, and amylase activity tests. Genomic DNA was extracted from purified isolates, and …
Anahtar Kelimeler
16S rRNA | Bacterial diversity | Enzymatic activity | Hospital infections | Phylogenetic analysis | Septic environment