| Yazarlar |
Hasan Türkez
Erzurum Teknik Üniversitesi, Türkiye |
|
Fatime Geyikoğlu
Atatürk Üniversitesi, Türkiye |
|
Mokhtar İbrahim Yousef
Türkiye |
|
Başak Toğar
Bayburt Üniversitesi, Türkiye |
|
Hasan Gürbüz
Atatürk Üniversitesi, Türkiye |
|
Kübra Çelik
Recep Tayyip Erdoğan Üniversitesi, Türkiye |
Doç. Dr. Giray Buğra AKBABA
Kafkas Üniversitesi, Türkiye |
|
Zuhal Polat
Türkiye |
| Özet |
| The purpose of this study was to evaluate the effect of carotenoid astaxanthin (ASTA) on cultured primary rat hepatocytes treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on the cell viability (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, MTT), lactate dehydrogenase (LDH) activity, 8-oxo-2-deoxyguanosine (8-OH-dG), total antioxidant capacity (TAC), and total oxidative stress (TOS) levels, and liver micronucleus rates. ASTA (2.5, 5, and 10 µM) was added to cultures alone or simultaneously with TCDD (5 and 10 µM) for 48 h. The results of MTT and LDH assays showed that both doses of TCDD caused significant decrease in cell viability. Also, TCDD significantly increased TOS and decreased TAC level in rat hepatocytes. On the basis of increasing doses, the dioxin caused significant increase in micronucleated hepatocytes) and 8-OH-dG level as compared to control culture. The presence of ASTA with TCDD minimized its effects on primary hepatocytes cultures and DNA damages. |
| Anahtar Kelimeler |
| TCDD,astaxanthin,cultured rat hepatocytes,cell viability,genotoxicity,oxidative status |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | Toxicology and Industrial Health |
| Dergi ISSN | 0748-2337 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Makale Dili | İngilizce |
| Basım Tarihi | 02-2014 |
| Cilt No | 30 |
| Sayı | 2 |
| Sayfalar | 101 / 112 |
| Doi Numarası | 10.1177/0748233712452607 |
| Atıf Sayıları | |
| WoS | 15 |
