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Effect of cobalt hydroxybenzoate complex on genomic DNA      
Yazarlar
A. Kadir Devrim
Kafkas Üniversitesi, Turkey
Ali Arslantas
Kafkas Üniversitesi, Turkey
Necati Kaya
Kafkas Üniversitesi, Turkey
Prof. Dr. Hacali NECEFOĞLU Prof. Dr. Hacali NECEFOĞLU
Kafkas Üniversitesi, Türkiye
Özet
In this study, the interaction between [Co(H2o)6] (p-HO-C6H4COO)2·2H2O complex and genomic DNA were investigated. DNA samples have been isolated from cattle leucocytes and the investigation carried out by incubating samples with the complex at 37°C. The effect of the metal complex on genomic DNA was ascertained by agarose gel electrophoresis at 50 V for 2 h. The intensity of the bands on the gel depends on the concentrations of the complex. The mobility of the bands decreased as the concentration of complex increased, indicating the occurrence of increased covalent binding of the metal complex with DNA. The damage effect of the added ascorbate into the medium might be depend on the free radicals produced from oxidation of ascorbate by molecular oxygen and this damage was reduced by the metal complex.
Anahtar Kelimeler
DNA damage | gel glectrophoresis | genomic DNA | cobalt | complex
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı ASIAN JOURNAL OF CHEMISTRY
Dergi ISSN 0970-7077
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce
Basım Tarihi 05-2007
Cilt No 19
Sayı 3
Sayfalar 2374 / 2380
BM Sürdürülebilir Kalkınma Amaçları
Atıf Sayıları
WoS 1
SCOPUS 1

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