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An investigation on the effects of Nickel II p hydroxybenzoate on genomic DNA      
Yazarlar
A. Kadir Devrim
Kafkas Üniversitesi, Turkey
Ali Arslantas
Kafkas Üniversitesi, Turkey
Necati Kaya
Kafkas Üniversitesi, Turkey
Prof. Dr. Hacali NECEFOĞLU Prof. Dr. Hacali NECEFOĞLU
Kafkas Üniversitesi, Türkiye
Özet
In this study, an investigation was performed to determine the interaction between genomic DNA and Ni(II) p-hydroxybenzoate complex. DNA samples have been isolated from the cattle leucocytes and the investigation was carried out by incubating the samples with the Ni complex at 37°C for varying times. The effect of the Ni(II) complex on genomic DNA was carried out by agarose gel electrophoresis at 50 V for 2 h. The intensity of the bands on the gel changed due to varied concentrations of the complex. The mobility of the bands declined as the concentrations of complex increased, indicating the occurrence of increased covalent binding of the metal complex with DNA. The damage effect of the added ascorbate into the medium might be dependent on the free radicals produced from oxidation of ascorbate by molecular oxygen and this damage is considered to reduced by the metal complex.
Anahtar Kelimeler
DNA damage | sheep genomic DNA | nickel complex | gel electrophoresis | UV spectra
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı ASIAN JOURNAL OF CHEMISTRY
Dergi ISSN 0970-7077
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce
Basım Tarihi 12-2007
Cilt No 19
Sayı 7
Sayfalar 5417 / 5424
BM Sürdürülebilir Kalkınma Amaçları
Atıf Sayıları
WoS 1
SCOPUS 1

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